microRNA in situ hybridization for miR-211 detection as an ancillary test in melanoma diagnosis
microRNA in situ hybridization for miR-211 detection as an ancillary test in melanoma diagnosis
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DOI：
10.1038/modpathol.2016.44
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发表时间：
2016-05-01
期刊：
影响因子：
7.5
通讯作者：
Dadras, Soheil S.
中科院分区：
文献类型：
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作者：
Babapoor, Sankhiros;Horwich, Michael;Dadras, Soheil S.
一些黑素细胞肿瘤在组织学上可能不明确，导致诊断困难，这可能导致对良性病变的过度治疗以及不必要的心理困扰，或者对恶性肿瘤的治疗不足。先前，我们证明与黑素细胞痣相比，黑素瘤中miR - 211的表达显著降低，能够准确区分恶性肿瘤和良性肿瘤。在此，我们展示了用于miR - 211荧光检测的原位微小RNA杂交技术，该技术适用于109例原发性黑素细胞肿瘤的石蜡包埋组织。与痣相比，黑素瘤中miR - 211的表达显著更低（P
Some melanocytic tumors can be histologically ambiguous causing diagnostic difficulty, which could lead to overtreatment of benign lesions with an unwarranted psychological distress or undertreatment of malignant cancers. Previously, we demonstrated that significantly decreased miR-211 expression in melanomas compared with melanocytic nevi could accurately discriminate malignant from benign tumors. Herein we show microRNA in situ hybridization for fluorescent detection of miR-211, suitable for paraffin-embedded tissues in 109 primary melanocytic tumors. miR-211 expression was significantly lower in melanomas vs nevi (P