Neurogenic potential of progenitor cells isolated from postmortem human Parkinsonian brains.
Neurogenic potential of progenitor cells isolated from postmortem human Parkinsonian brains.
复制标题
从死后人类帕金森病大脑中分离出的祖细胞的神经发生潜力。
DOI：
10.1016/j.brainres.2012.04.039
复制
发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
Steindler,DennisA
中科院分区：
文献类型：
--
作者：
Wang,Shanshan;Okun,MichaelS;Suslov,Oleg;Zheng,Tong;McFarland,NikolausR;Vedam-Mai,Vinata;Foote,KellyD;Roper,StevenN;Yachnis,AnthonyT;Siebzehnrubl,FlorianA;Steindler,DennisA
帕金森病（PD）细胞疗法的成功不仅取决于体内有利的生长环境，而且还取决于干/祖细胞的离体扩增和靶向神经分化。在这里，我们证明了干细胞/祖细胞、从特发性PD死后组织样品中分离的成人神经祖细胞（“AHNP”）以及在较小程度上丢弃的深部脑刺激电极的体外增殖和分化潜力。我们证明，这些AHNP可以从许多结构中分离出来，（例如黑质，“SN”），并且能够分化成神经胶质和神经元，但仅在特定的生长条件下，包括与胚胎干细胞衍生的神经前体（“ESNP”）共培养;这表明PD多能神经干/祖细胞确实存在于SN和其它区域内，但其本身似乎缺乏神经元分化所需的关键因子。AHNP在离体扩增和移植到啮齿动物脑中后植入，证明了它们的再生潜力。我们的数据证明了PD脑中内源性干/祖细胞的存在和能力。
The success of cellular therapies for Parkinson's disease (PD) will depend not only on a conducive growth environment in vivo, but also on the ex vivo amplification and targeted neural differentiation of stem/progenitor cells. Here, we demonstrate the in vitro proliferative and differentiation potential of stem/progenitor cells, adult human neural progenitor cells (“AHNPs”) isolated from idiopathic PD postmortem tissue samples and, to a lesser extent, discarded deep brain stimulation electrodes. We demonstrate that these AHNPs can be isolated from numerous structures (e.g. substantia nigra, “SN”) and are able to differentiate into both glia and neurons, but only under particular growth conditions including co-culturing with embryonic stem cell-derived neural precursors (“ESNPs”); this suggests that PD multipotent neural stem/progenitor cells do reside within the SN and other areas, but by themselves appear to lack key factors required for neuronal differentiation. AHNPs engraft following ex vivo expansion and transplantation into the rodent brain, demonstrating their regenerative potential. Our data demonstrate the presence and capacity of endogenous stem/progenitor cells in the PD brain.