In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation.
In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation.
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DOI：
10.1117/1.2939401
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发表时间：
2008-05
影响因子：
3.5
通讯作者：
W. Watanabe;S. Matsunaga;T. Higashi;K. Fukui;K. Itoh
中科院分区：
文献类型：
--
作者：
W. Watanabe;S. Matsunaga;T. Higashi;K. Fukui;K. Itoh
近红外区的飞秒激光脉冲在细胞内细胞器的成像和操纵方面具有潜在的应用。我们报道了双光子激发对细胞内细胞器的操纵。用光漂白后双光子荧光恢复(FRAP)法研究了绿色荧光蛋白(GFP)-组蛋白的动力学。通过聚焦飞秒激光脉冲对活细胞内的荧光标记细胞器进行细胞内消融。我们报道了利用光可转化荧光蛋白的双光子转换对单个细胞器进行选择性标记。
Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein.