Menadione-induced oxidative stress accelerates onset of Emory mouse cataract in vivo.
Menadione-induced oxidative stress accelerates onset of Emory mouse cataract in vivo.
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甲萘醌诱导的氧化应激加速埃默里小鼠体内白内障的发生。
DOI：
10.1076/ceyr.16.6.519.5070
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发表时间：
1997
影响因子：
2
通讯作者：
Bhuyan,KC
中科院分区：
文献类型：
--
作者：
Bhuyan,DK;Huang,X;Kuriakose,G;Garner,WH;Bhuyan,KC
PURPOSEWe evaluated the effect of the free-radical generator, menadione, on the time of occurrence of cataract in the Emory mouse, a model for human cataract. Concomitant experiments were done to compare production and level of reactive metabolites of oxygen in the Emory mouse and its cataract resistant (CR) genetic control.METHODSTest and control mice were fed both a normal diet and a diet supplemented with menadione, and the lenses were evaluated for the time of occurrence of cataract and the level of membrane ATPases. Effects of menadione were determined on incubated lenses of Emory and CR mice, assaying reactive species of oxygen, levels of antioxidant enzymes, and formation of the lipid peroxidation product, malondialdehyde.RESULTSSystemic administration of menadione markedly accelerated the onset of Emory mouse cataract, and decreased ATPase activities suggested oxidative damage to membrane proteins. Cumulative levels of O 2 · -, H 2 O 2, ·OH and malondial-dehyde were significantly higher than controls in the lenses incubated in the presence of menadione, showing that it generates oxidative stress. However, [GSH] in lenses decreased equally in test and control mice. The observed increases in catalase and glutathione peroxidase activities in the test lenses indicated an early protective response to oxidative insult.CONCLUSIONSAcceleration by menadione of the appearance of cataract in the Emory mouse demonstrates that oxidative stress is a risk factor in late-onset cataract. This quinone caused a greater increase in the production and levels of reactive metabolites of oxygen in Emory mice than in CR mice, indicative of a higher susceptibility of the former to oxidative insult.
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埃默里小鼠白内障：人类老年性白内障的动物模型。
影响因子：
2
作者：
J. Kuck;Toichiro Kuwabara;K. Kuck
通讯作者：
K. Kuck
从进食和禁食大鼠中分离出的肝细胞中甲萘醌诱导的氧化应激：NADPH 再生途径的作用。
影响因子：
3.8
作者：
P. F. Smith;D. Alberts;G. Rush
通讯作者：
G. Rush
DOI：
10.1042/bj1020842
发表时间：
1967
期刊：
The Biochemical journal
影响因子：
--
作者：
R. van Heyningen;A. Pirie
通讯作者：
R. van Heyningen;A. Pirie
埃默里小鼠白内障：可溶性蛋白丢失、谷胱甘肽、蛋白巯基等变化。
影响因子：
3.4
作者：
J. Kuck;K. Kuck
通讯作者：
K. Kuck
DOI：
10.1073/pnas.80.7.2044
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GARNER, WH;GARNER, MH;SPECTOR, A
通讯作者：
SPECTOR, A