Survival of Helicobacter pylori in a natural freshwater environment
Survival of Helicobacter pylori in a natural freshwater environment
复制标题
DOI：
10.1128/aem.69.12.7462-7466.2003
复制
发表时间：
2003-12-01
影响因子：
4.4
通讯作者：
Oliver, JD
中科院分区：
文献类型：
--
作者：
Adams, BL;Bates, TC;Oliver, JD
大多数胃溃疡的病原体幽门螺杆菌的传播方式仍未确定。流行病学证据表明，这些生物是水传播的；然而，幽门螺杆菌很少从潜在的水源中生长。这可能是由于这种生物能够迅速进入可存活但不可培养（VBNC）状态。我们的研究考察了幽门螺杆菌在实验室培养和自然淡水环境中进入这种状态的入口，以及形态和可培养性之间的关系。为此，利用膜扩散室将细胞暴露于淡水流的自然波动中。在实验室和环境中，使用平板计数对样品进行培养性检测，并使用LIVE/DEAD BacLight染色法进行活力和形态学检测。此外，采集水样，测量6个环境参数，并检查复苏条件。在实验室和流水中观察到幽门螺杆菌失去了培养能力，尽管生存能力保持不变。虽然我们的研究结果与之前的研究结果一致，即当可培养性丧失时，细胞的形态会从杆状体转变为球菌，但细胞的形态分布并没有随着环境中可培养性的丧失而改变。实验室中处于VBNC状态的细胞以球菌为主；然而，所有形态形式都存在于环境中。这些研究结果表明，幽门螺杆菌在实验室培养物和VBNC状态下的环境中持续存在，这种状态下的细胞对公众健康构成危害。
The mode by which Helicobacter pylori, the causative agent of most gastric ulcers, is transmitted remains undetermined. Epidemiological evidence suggests these organisms are waterborne; however, H. pylori has rarely been grown from potential water sources. This may be due to the ability of this organism to rapidly enter the viable but nonculturable (VBNC) state. Our investigation examines the entrance of H. pylori into this state in laboratory cultures and a natural freshwater environment as well as the relationship between morphology and culturability. To this end, membrane diffusion chambers were utilized to expose the cells to the natural fluctuations of a freshwater stream. In both the laboratory and environment, samples were assayed for culturability using plate counts and stained using a LIVE/DEAD BacLight assay for viability and morphological determinations. Additionally, water samples were collected, six environmental parameters were measured, and resuscitation conditions were examined. H. pylori was observed to lose culturability in the laboratory and stream, although viability was maintained. While the results of our study agree with those of previous studies which suggested that there is a transition in morphology from rods to cocci as culturability is lost, the morphological distribution of cells did not change as culturability was lost in the environment. The majority of cells in the VBNC state in the laboratory are cocci; however, all morphological forms were present in the environment. The results of these studies suggest that H. pylori persists in laboratory cultures and the environment in the VBNC state and that cells in this state represent a public health hazard.