Anti-interferon gamma treatment blocks the ability of glutaraldehyde-polymerized allergens to inhibit specific IgE responses.
Anti-interferon gamma treatment blocks the ability of glutaraldehyde-polymerized allergens to inhibit specific IgE responses.
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DOI：
10.1084/jem.173.2.279
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发表时间：
1991-02-01
期刊：
影响因子：
--
通讯作者：
Stefura BP
中科院分区：
文献类型：
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作者：
HayGlass KT;Stefura BP
淋巴因子白介素4和干扰素-γ在体内外对多克隆免疫球蛋白E(IgE)和IgG2a反应的调节中起着重要作用。我们在这里证明，用化学修饰的卵清蛋白(OA)治疗可导致97-99%的变态反应原特异性小鼠IgE反应被长期抑制，抗OA IgG2a抗体增加10(3)-10(4)倍。对无关抗原的反应不受影响。在相同条件下，未经修饰的OA治疗不能抑制初次或继发性IgE反应，也不能增加IgG2a，但确实导致OA特异性IgG1的显著增加。戊二醛聚合卵清蛋白(OA-POL)诱导的IgE和IgG2a反应的变化可通过纯化的单抗干扰素-γ抗体(XMG 1.2)在体内治疗而被取消，这一发现表明，暴露于化学修饰的但不是天然的过敏原时，优先产生干扰素-γ。结果表明，暴露于蛋白质抗原后引发的细胞因子合成模式以及由此产生的免疫反应可能取决于个体接触的抗原形式，因此可能受到操纵。
The lymphokines interleukin 4 and interferon gamma (IFN-gamma) have been shown to play an important role in regulation of polyclonal immunoglobulin E (IgE) and IgG2a responses in vitro and in vivo. We demonstrate here that treatment with chemically modified ovalbumin (OA) results in long-lived, 97-99% inhibition of allergen-specific murine IgE responses and 10(3)-10(4)-fold increases in anti-OA IgG2a. Responses to unrelated antigens are not affected. Treatment with unmodified OA under the same conditions fails to inhibit primary or secondary IgE responses or to increase IgG2a but does lead to pronounced increases in OA-specific IgG1 production. Glutaraldehyde- polymerized ovalbumin (OA-POL)-induced changes in IgE and IgG2a responses are abrogated by in vivo treatment with purified monoclonal anti-IFN-gamma antibody (XMG 1.2), a finding indicative of preferential IFN-gamma production upon exposure to chemically modified, but not native, allergen. The results suggest the possibility that the pattern of cytokine synthesis elicited after exposure to protein antigens, and the resulting immune response, may be dependent upon the form of antigen to which the individual is exposed and consequently may be subject to manipulation.