A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy.
A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy.
复制标题
通过光子谐振器吸收显微镜数字检测外泌体 MicroRNA 的目标回收放大过程。
DOI：
10.1002/anie.202217932
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Cunningham,BrianT
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文献类型：
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作者：
Wang,Xiaojing;Shepherd,Skye;Li,Nantao;Che,Congnyu;Song,Tingjie;Xiong,Yanyu;Palm,IsabellaRose;Zhao,Bin;Kohli,Manish;Demirci,Utkan;Lu,Yi;Cunningham,BrianT
外泌体microRNA（miRNAs）作为监测癌症发展、疾病进展、治疗效果和预后的关键生物标志物具有相当大的潜力。在这里，我们报告了一种高效的目标回收放大过程（TRAP），用于使用光子谐振器吸收显微镜数字检测miRNA。我们在20分钟内实现了亚阿摩尔灵敏度的多重数字检测，对单核苷酸变体具有强大的选择性，以及从1 aM到1 pM的宽动态范围。 与传统的qRT-PCR相比，TRAP在分析来自癌细胞的外泌体miRNA方面显示出相似的准确性，但在miRNA-375和miRNA-21的检测极限方面也分别表现出至少31倍和61倍的增强。TRAP方法是外泌体或循环miRNA生物标志物定量的理想方法，其中miRNA以低浓度或样品体积存在，具有频繁，低成本和微创床旁检测的潜力。
Exosomal microRNAs (miRNAs) have considerable potential as pivotal biomarkers to monitor cancer development, dis‐ease progression, treatment effects and prognosis. Here, we report an efficient target recycling amplification process (TRAP) for the digital detection of miRNAs using photonic resonator absorption microscopy. We achieve multiplex digital detection with sub‐attomolar sensitivity in 20 minutes, robust selectivity for single nucleotide variants, and a broad dynamic range from 1 aM to 1 pM. Compared with traditional qRT‐PCR, TRAP showed similar accuracy in profiling exosomal miRNAs derived from cancer cells, but also exhibited at least 31‐fold and 61‐fold enhancement in the limits of miRNA‐375 and miRNA‐21 detection, respectively. The TRAP approach is ideal for exosomal or circulating miRNA biomarker quantification, where the miRNAs are present in low concentrations or sample volume, with potentials for frequent, low‐cost, and minimally invasive point‐of‐care testing.