Oscillating global regulators control the genetic circuit driving a bacterial cell cycle
Oscillating global regulators control the genetic circuit driving a bacterial cell cycle
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DOI：
10.1126/science.1095191
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发表时间：
2004-05-14
期刊：
影响因子：
56.9
通讯作者：
Shapiro, L
中科院分区：
文献类型：
--
作者：
Holtzendorff, J;Hung, D;Shapiro, L
一种新发现的细胞周期主调控蛋白GcrA和CtrA主调控蛋白是驱动新月茎杆菌细胞周期进程和不对称极性形态发生的遗传回路的关键组成部分。该电路驱动GcrA和CtrA浓度的非相位时空振荡，对实现细胞周期过程的模块功能产生依赖于时间和空间的转录调节。CtrA/GcrA调控回路控制极性分化因子的表达和DNA复制的时间。CtrA是复制起始点的消声器，而GcrA是复制体和分离机制组分的激活剂。
A newly identified cell-cycle master regulator protein, GcrA, together with the CtrA master regulator, are key components of a genetic circuit that drives cell-cycle progression and asymmetric polar morphogenesis in Caulobacter crescentus. The circuit drives out-of-phase temporal and spatial oscillation of GcrA and CtrA concentrations, producing time- and space-dependent transcriptional regulation of modular functions that implement cell-cycle processes. The CtrA/GcrA regulatory circuit controls expression of polar differentiation factors and the timing of DNA replication. CtrA functions as a silencer of the replication origin and GcrA as an activator of components of the replisome and the segregation machinery.