Characterization of the genes, including that encoding the viral proteinase, contained in BamHI restriction fragment 9 of the pseudorabies virus genome
Characterization of the genes, including that encoding the viral proteinase, contained in BamHI restriction fragment 9 of the pseudorabies virus genome
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DOI：
10.1099/0022-1317-77-8-1865
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发表时间：
1996-08-01
影响因子：
3.8
通讯作者：
Tabares, E
中科院分区：
文献类型：
--
作者：
Camacho, A;Tabares, E
我们描述了位于BamHI限制性片段9(0.406-0。在伪狂犬病病毒(PRV)基因组唯一的长片段中，该片段包含三个嵌套基因，它们都有一个共同的3‘端，对应的0.9、1.7和3.3kb的mRNAs已被定位，BamHI-9片段包含三个完整的ORF1、ORF2和ORF2.5。ORF1，它在3。3kb转录本，编码一个表观分子质量为60 kDa的蛋白质，它与单纯疱疹病毒1型UL25基因的产物1同源。7kb的mRNA含有ORF2，其产物与疱疹病毒蛋白水解酶同源，而0。9kb转录本含有ORF2.5，可能编码装配蛋白前体，以ORF2.5产物为底物蛋白，经鉴定为PRV蛋白水解酶基因ORF2。
We describe the nucleotide sequence, transcription pattern and open reading frames (ORFs) located on BamHI restriction fragment 9 (0 . 406-0 . 435 map units) in the unique long segment of the pseudorabies virus (PRV) genome, The fragment contains three nested genes with a common 3' end, The 5' ends of the corresponding 0 . 9, 1 . 7 and 3 . 3 kb mRNAs have been mapped, Fragment BamHI-9 contains three complete ORFs, ORF1, ORF2 and ORF2.5. ORF1, which is within the 3 . 3 kb transcript, encodes a protein with an apparent molecular mass of 60 kDa which is homologous to the product of the herpes simplex virus type 1 UL25 gene, The 1 . 7 kb mRNA contains ORF2, whose product is homologous to the herpesvirus proteinases, while the 0 . 9 kb transcript contains ORF2.5, which probably encodes the assembly protein precursor, ORF2 was identified as the PRV proteinase gene following expression in E. coli, using the product of ORF2.5 as the substrate protein.