Correlation of invasion and metastasis of cancer cells, and expression of the RAD21 gene in oral squamous cell carcinoma
Correlation of invasion and metastasis of cancer cells, and expression of the RAD21 gene in oral squamous cell carcinoma
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DOI：
10.1007/s00428-005-0132-y
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发表时间：
2006-04-01
期刊：
影响因子：
3.5
通讯作者：
Tachikawa, T
中科院分区：
文献类型：
--
作者：
Yamamoto, G;Irie, T;Tachikawa, T
尽管RAD21参与DNA双链断裂的修复，对有丝分裂的生长是必不可少的，但它在癌症中的作用尚不清楚。本研究利用激光显微切割技术和实时定量聚合酶链式反应技术，探讨RAD21基因表达与口腔鳞癌侵袭转移的关系。以两种不同转移潜能的口腔鳞状细胞[高转移潜能鳞癌细胞(SAS-Ly)和低转移潜能鳞癌细胞(SAS)]为研究对象，探讨RAD21基因表达与口腔鳞癌细胞凋亡、侵袭和转移的关系。结果表明，口腔鳞状细胞癌组织中RAD21基因表达水平明显低于干扰素α侵袭模式(p<；0.01)。此外，与SAS细胞相比，SAS-Ly细胞表现出对凋亡诱导剂诱导的细胞死亡的耐受性，而SAS细胞中RAD21基因的表达水平则被凋亡诱导剂上调。然而，在SAS-Ly细胞中，该试剂诱导的差异不显著。我们的发现表明RAD21基因与癌细胞的侵袭和转移密切相关。
Although RAD21 is involved in the repair of double-strand breaks in DNA and is essential for mitotic growth, its role in cancer has been unclear. In this study, the relevance of RAD21 gene expression to the invasion and metastasis of oral squamous cell carcinoma was clarified using laser microdissection and real-time polymerase chain reaction (PCR). Using two different metastatic potential oral squamous cells [high-metastatic-potential squamous cell carcinoma cells (SAS-Ly) and low-metastatic-potential squamous cell carcinoma cells (SAS)], the relation of RAD21 gene expression to apoptosis, invasion, and metastasis was examined. The results showed that RAD21 gene expression was significantly decreased in oral squamous cell carcinoma when it expressed the INF beta and INF gamma invasion patterns in comparison with the INF alpha invasion pattern (p < 0.01). In addition, in comparison with SAS cells, SAS-Ly cells indicated tolerance to cell death induced by an apoptosis induction reagent, while the expression level of the RAD21 gene in SAS cells was increased by the apoptosis induction reagent. However, in SAS-Ly cells, the reagent induced no significant difference. Our findings indicate that the RAD21 gene was closely related to the invasion and metastasis of cancer cells.