Human extracellular superoxide dismutase (EC-SOD) expression in transgenic chicken.
Human extracellular superoxide dismutase (EC-SOD) expression in transgenic chicken.
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DOI：
10.5483/bmbrep.2013.46.8.251
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发表时间：
2013-08
期刊：
影响因子：
3.8
通讯作者：
Kim TY
中科院分区：
文献类型：
--
作者：
Byun SJ;Ji MR;Jang YJ;Hwang AI;Chung HK;Kim JS;Kim KW;Chung HJ;Yang BC;Jeon I;Park JK;Yoo JG;Kim TY
细胞外超氧化物歧化酶（EC-SOD）是一种金属蛋白，具有抗氧化酶的功能。在这项研究中，我们使用慢病毒载体，以产生转基因鸡，表达人类EC-SOD基因。将重组慢病毒注射到新鲜产蛋的胚下腔中。随后，使用替代壳离体培养系统的阶段II和III孵育胚胎以孵化。在158个注射胚胎中，16只鸡（G 0）孵化，并通过PCR筛选hEC-SOD。只有1只鸡被鉴定为在其生殖系中含有转基因的转基因鸟。将该建立者（G 0）鸟与野生型母鸡交配以产生转基因后代，并产生2只转基因小鸡（G1）。在所产生的转基因母鸡（G2）中，hEC-SOD蛋白在鸡蛋白色中表达并显示抗氧化活性。这些结果突出了鸡在鸡蛋白色中生产生物活性蛋白质的潜力。[BMB报告2013; 46（8）：404-409]
Extracellular superoxide dismutase (EC-SOD) is a metalloprotein and functions as an antioxidant enzyme. In this study, we used lentiviral vectors to generate transgenic chickens that express the human EC-SOD gene. The recombinant lentiviruses were injected into the subgerminal cavity of freshly laid eggs. Subsequently, the embryos were incubated to hatch using phases II and III of the surrogate shell ex vivo culture system. Of 158 injected embryos, 16 chicks (G0) hatched and were screened for the hEC-SOD by PCR. Only 1 chick was identified as a transgenic bird containing the transgene in its germline. This founder (G0) bird was mated with wild-type hens to produce transgenic progeny, and 2 transgenic chicks (G1) were produced. In the generated transgenic hens (G2), the hEC-SOD protein was expressed in the egg white and showed antioxidant activity. These results highlight the potential of the chicken for production of biologically active proteins in egg white. [BMB Reports 2013; 46(8): 404-409]
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影响因子：
5.6
作者：
Antonyuk, Svetlana V.;Strange, Richard W.;Hasnain, S. Samar
通讯作者：
Hasnain, S. Samar
DOI：
10.1073/pnas.93.13.6659
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