The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection.
The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection.
复制标题
大鼠大神经丝亚基（NF-H）：cDNA 克隆和原位检测。
DOI：
10.1016/0006-291x(88)90254-9
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
P. Jollés
中科院分区：
文献类型：
--
作者：
A. Dautigny;D. Pham‐Dinh;C. Roussel;J. Félix;J. Nussbaum;P. Jollés
用2′，3′-环核苷酸3′-磷酸水解酶对应的Wla Wolfgram蛋白多克隆抗血清筛选了λgtll表达文库。这种抗血清也能识别神经丝高蛋白亚基（NF-H）的表位。分离得到NF-H cDNA克隆（pNF-H1: 1.7 kb）并对其进行了鉴定。以pNF-H1为探针，从λgtll文库中分离到第二个cDNA克隆（pNF-H2: 3 kb）。对这两个克隆进行测序，发现pNF-H2编码80%的大鼠NF-H蛋白（线圈-2和c端区）。c端区域包含一个不寻常的序列，其中包含Lys-Ser-Pro的重复片段，代表可能的磷酸化位点。以pNF-H1为探针，原位杂交证实了相应mRNA在神经元中的特异性定位。
A λgtll expression library was screened with a polyclonal antiserum directed against Wla Wolfgram protein which corresponds to the 2′,3′-cyclic nucleotide 3′-phosphohydrolase. This antiserum also recognizes epitopes of the high protein subunit of neurofilaments (NF-H). An NF-H cDNA clone (pNF-H1: 1.7 kb) was isolated and characterized. Using pNF-H1 as a probe, a second cDNA clone (pNF-H2: 3 kb) was isolated from the λgtll library. The two clones were sequenced and pNF-H2 was found to encode 80% of rat NF-H protein (coil-2 and C-terminal region). The C-terminal region contains an unusual sequence with stretches of repeats of Lys-Ser-Pro which represent possible phosphorylation sites. Specific localization in neurons of the corresponding mRNA was demonstrated by in situ hybridization using the pNF-H1 as a probe.
神经丝周围区域的结构、生化特性和免疫原性由磷酸化状态决定。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Carden,MJ;Schlaepfer,WW;Lee,VM
通讯作者：
Lee,VM