Molecular characterization of two isoforms of ZFAND3 cDNA from the Japanese quail and the leopard gecko, and different expression patterns between testis and ovary
Molecular characterization of two isoforms of ZFAND3 cDNA from the Japanese quail and the leopard gecko, and different expression patterns between testis and ovary
复制标题
日本鹌鹑和豹纹守宫两种 ZFAND3 cDNA 亚型的分子特征，以及睾丸和卵巢之间的不同表达模式
DOI：
10.1016/j.gene.2011.08.021
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发表时间：
2011
期刊：
影响因子：
3.5
通讯作者：
& Park MK
中科院分区：
文献类型：
--
作者：
Otake S;Endo D;& Park MK
Zing finger AN 1-type domain 3（ZFAND 3）又称睾丸表达序列27（Tex 27），是一个存在于小鼠睾丸中的基因，但其生理功能尚不清楚。我们鉴定了来自日本鹌鹑和豹壁虎的ZFAND 3 cDNA的两种亚型（短型和长型）的全长序列。这是首次克隆鸟类和爬行动物ZFAND 3 cDNA。这两种亚型通过3′UTR中的交替多聚腺苷酸化产生，并且具有编码相同蛋白的相同ORF序列。从哺乳动物到两栖动物，长型的3′UTR在多聚腺苷酸化位点附近都存在高度保守的区域，表明不同亚型之间决定mRNA稳定性或翻译效率的特征不同。ZFAND 3的推导氨基酸序列具有两个锌指结构域，N端的A20样锌指结构域和C端的AN 1样锌指结构域。序列分析显示，在鸟类和爬行动物ZFAND 3基因的基因组结构中存在一个额外的外显子，该外显子在哺乳动物、两栖动物或鱼类中不存在，并且该外显子在A20样锌指结构域中产生额外的氨基酸残基。在日本鹌鹑中的表达分析表明，ZFAND 3 mRNA不仅在睾丸中表达，而且在卵巢中也有高水平的表达，并且ZFAND 3 mRNA在睾丸的精子和卵巢的卵母细胞中都有表达。短型mRNA主要在精巢中表达，长型mRNA在卵巢中表达量较高。这些结果表明，ZFAND 3具有与生殖细胞成熟相关的生理功能和睾丸和卵巢之间不同的调节机制。
Zing finger AN1-type domain 3 (ZFAND3), also known as testis expressed sequence 27 (Tex27), is a gene found in the mouse testis, but its physiological function is unknown. We identified the full-length sequences of two isoforms (short and long) of ZFAND3 cDNA from Japanese quail and leopard gecko. This is the first cloning of avian and reptilian ZFAND3 cDNA. The two isoforms are generated by alternative polyadenylation in the 3′UTR and have the same ORF sequences encoding identical proteins. There were highly conserved regions in the 3′UTR of the long form near the polyadenylation sites from mammals to amphibians, suggesting that the features for determining the stability of mRNA or translation efficiency differ between isoforms. The deduced amino acid sequence of ZFAND3 has two putative zinc finger domains, an A20-like zinc finger domain at the N-terminal and an AN1-like zinc finger domain at the C-terminal. Sequence analysis revealed an additional exon in the genomic structures of the avian and reptilian ZFAND3 genes which is not present in mammals, amphibians, or fish, and this exon produces additional amino acid residues in the A20-like zinc finger domain. Expression analysis in Japanese quail revealed that the expression level of ZFAND3 mRNA was high in not only the testis but also the ovary, and ZFAND3 mRNA was expressed in both spermatides of the testis and oocytes of the ovary. While the short form mRNA was mainly expressed in the testis, the expression level of the long form mRNA was high in the ovary. These results suggest that ZFAND3 has physiological functions related to germ cell maturation and regulatory mechanisms that differ between the testis and ovary.