Activation of hypodermal differentiation in the Caenorhabditis elegans embryo by GATA transcription factors ELT-1 and ELT-3
Activation of hypodermal differentiation in the Caenorhabditis elegans embryo by GATA transcription factors ELT-1 and ELT-3
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DOI：
10.1128/mcb.21.7.2533-2544.2001
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发表时间：
2001-04-01
影响因子：
5.3
通讯作者：
McGhee, JD
中科院分区：
文献类型：
--
作者：
Gilleard, JS;McGhee, JD
秀丽隐杆线虫加塔转录因子基因elt-1和elt-3在胚胎皮下组织（也称为表皮）中表达。ELT-1在前体细胞中表达，并且对于大多数皮下细胞的产生是必需的（22）。ELT-3在除侧缝细胞外的所有主要皮下细胞中表达，并且表达在前体谱系的终末分裂后立即开始（13）。虽然这种表达模式表明ELT-3在皮下发育中的作用，但尚未进行功能研究。在本文中，我们表明，无论是ELT-3或ELT-1是足够的，当力在早期胚胎卵裂球中表达，激活程序的真皮分化，即使在卵裂球，不是真皮前体在野生型胚胎。我们已经删除了ELT-3基因，并表明ELT-3对皮下细胞分化或生物体的生存能力都不是必需的。我们发现，ELT-3可以激活皮下基因表达的ELT-1的情况下，相反，ELT-1可以激活皮下基因表达的ELT-3的情况下。总之，突变体表型、初始表达时间和我们的强制表达实验的综合结果表明，ELT-3在控制皮下细胞分化的冗余途径中作用于ELT-1的下游。
The Caenorhabditis elegans GATA transcription factor genes elt-1 and elt-3 are expressed in the embryonic hypodermis (also called the epidermis). elt-l is expressed in precursor cells and is essential for the production of most hypodermal cells (22). elt-3 is expressed in all of the major hypodermal cells except the lateral seam cells, and expression is initiated immediately after the terminal division of precursor lineages (13). Although this expression pattern suggests a role for ELT-3 in hypodermal development, no functional studies have yet been performed. In the present paper, we show that either elt-3 or elt-1 is sufficient, when force expressed in early embryonic blastomeres, to activate a program of hypodermal differentiation even in blastomeres that are not hypodermal precursors in wild-type embryos. We have deleted the elt-3 gene and shown that ELT-3 is not essential for either hypodermal cell differentiation or the viability of the organism. We showed that ELT-3 can activate hypodermal gene expression in the absence of ELT-1 and that, conversely, ELT-1 can activate hypodermal gene expression in the absence of ELT-3. Overall, the combined results of the mutant phenotypes, initial expression times, and our forced-expression experiments suggest that ELT-3 acts downstream of ELT-1 in a redundant pathway controlling hypodermal cell differentiation.