Bacterial self-defense antibiotics release from organic-inorganic hybrid multilayer films for long-term anti-adhesion and biofilm inhibition properties
Bacterial self-defense antibiotics release from organic-inorganic hybrid multilayer films for long-term anti-adhesion and biofilm inhibition properties
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DOI：
10.1039/c7nr07106j
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发表时间：
2017-12-28
期刊：
影响因子：
6.7
通讯作者：
Wang, Bailiang
中科院分区：
文献类型：
--
作者：
Xu, Qingwen;Li, Xi;Wang, Bailiang
植入物相关的细菌感染由于病原体在植入物表面的定植和增殖，引发了严重的医疗和经济问题。现有的传统抗菌表面既无法长期抵抗细菌黏附，也不能抑制生物膜的形成。在此，我们研发了新型的（蒙脱石/聚-L-赖氨酸-硫酸庆大霉素）₈（(MMT/PLL - GS)₈）有机 - 无机杂化多层膜，该膜结合了聚-L-赖氨酸的酶促降解特性，可按需实现抗生素的自防御性释放。在自组装过程中，小分子硫酸庆大霉素（GS）被载入多层膜中，且多层膜呈现出pH依赖和线性生长的特性。胰凝乳蛋白酶（CMS）和细菌感染会引发膜的降解，导致膜的剥落和GS的释放。通过抑菌圈大小和扫描电镜（SEM）图像测定，酶响应性GS释放呈现出对CMS浓度的依赖性。值得注意的是，所制备的抗菌膜展现出高效的杀菌活性，能在12小时内杀灭超过99.9%的金黄色葡萄球菌。即使在金黄色葡萄球菌、大肠杆菌或表皮葡萄球菌溶液中孵育3天后，多层膜仍能产生大于1.5毫米的抑菌圈。体外和体内抗菌测试均表明，该多层膜具有良好的细胞相容性、抗炎性，以及长期的细菌抗黏附和生物膜抑制性能。
Implant-associated bacterial infections pose serious medical and financial issues due to the colonization and proliferation of pathogens on the surface of the implant. The as-prepared traditional antibacterial surfaces can neither resist bacterial adhesion nor inhibit the development of biofilm over the long term. Herein, novel (montmorillonite/poly-L-lysine-gentamicin sulfate)(8) ((MMT/PLL-GS)(8)) organic-inorganic hybrid multilayer films were developed to combine enzymatic degradation PLL for on-demand self-defense antibiotics release. Small molecule GS was loaded into the multilayer films during self-assembly and the multilayer films showed pH-dependent and linear growth behavior. The chymotrypsin- (CMS) and bacterial infections-responsive film degradation led to the peeling of the films and GS release. Enzyme-responsive GS release exhibited CMS concentration dependence as measured by the size of the inhibition zone and SEM images. Notably, the obtained antibacterial films showed highly efficient bactericidal activity which killed more than 99.9% of S. aureus in 12 h. Even after 3 d of incubation in S. aureus, E. coli or S. epidermidis solutions, the multilayer films exhibited inhibition zones of more than 1.5 mm in size. Both in vitro and in vivo antibacterial tests indicated good cell compatibility, and anti-inflammatory, and long-term bacterial anti-adhesion and biofilm inhibition properties.