Assessment of antioxidant activity of eugenol in vitro and in vivo.
Assessment of antioxidant activity of eugenol in vitro and in vivo.
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DOI：
10.1007/978-1-60327-029-8_10
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Nakka, Lakshmaiah
中科院分区：
文献类型：
--
作者：
Nagababu, Enika;Rifkind, Joseph M.;Boindala, Sesikeran;Nakka, Lakshmaiah
活性氧与许多人类疾病和衰老过程有关。许多证据是基于实验数据，表明疾病状态下脂质过氧化率增加以及抗氧化剂的改善作用。越来越明显的是，具有酚类结构的天然抗氧化剂在保护组织免受自由基损伤方面发挥着重要作用。丁香酚（4-烯丙基-2甲氧基酚）是一种天然存在的酚类化合物。丁香酚的抗氧化活性是通过体外和体内研究对自由基介导的脂质过氧化提供保护的程度来评估的。（Fe(II)-抗坏血酸）或（Fe(II) + H2O2）在体外诱导线粒体脂质过氧化。脂质过氧化是通过测量氧化脂质与TBA反应后硫代巴比妥酸反应物质（TBARS）的形成来评估的。丁香酚抑制铁和Fenton试剂介导的脂质过氧化。丁香酚的抑制活性比α-生育酚高约5倍，比合成抗氧化剂BHT低约10倍。丁香酚的体内抗氧化活性是通过测定一些生化参数(SGOT, Cyt。P450，葡萄糖-6-磷酸酶)，过氧化产物和•CCl3自由基诱导大鼠肝毒性的组织病理学检查。丁香酚能显著抑制SGOT活性的升高和细胞坏死，而不保护内质网（ER）损伤，其不能阻止葡萄糖-6-磷酸酶活性的降低。丁香酚的保护作用已被发现是由于拦截来自内质网脂质的次级自由基，而不是干扰CCl4（•CCl3/ ccl300•）的初级自由基。
Reactive oxygen species are implicated in many human diseases and aging process. Much of the evidence is based on experimental data indicating increasing rates of lipid peroxidation in disease states and the ameliorating effects of antioxidants. It is becoming increasingly evident that the natural antioxidants, which have basically a phenolic structure, play an important role in protecting tissues against free radical damage. Eugenol (4-allyl-2 methoxyphenol), is one among such naturally occurring phenolic compounds. The antioxidant activity of eugenol is evaluated by the extent of protection offered against free radical mediated lipid peroxidation using both in vitro and in vivo studies. The in vitro lipid peroxidation is induced in mitochondria by (Fe(II)-ascorbate) or (Fe(II) + H2O2). The lipid peroxidation is assessed colorimetrically by measuring the formation of thiobarbituric acid reactive substances (TBARS) following the reaction of oxidized lipids with TBA. Eugenol inhibits both iron and Fenton reagent mediated lipid peroxidation. The inhibitory activity of eugenol is about five fold higher than α-tocopherol and about ten fold less than the synthetic antioxidant, BHT. The in vivo antioxidant activity of eugenol is evaluated by the determination of certain biochemical parameters (SGOT, Cyt.P450, glucose-6-phosphatase), peroxidation products and histopathological examination of •CCl3 radical induced hepatotoxicity in rats. Eugenol significantly inhibits the rise in SGOT activity and cell necrosis without protecting the endoplasmic reticulum (ER) damage as assessed by its failure to prevent a decrease in glucose-6-phosphatase activity. The protective action of eugenol has been found to be due to interception of secondary radicals derived from ER lipids rather than interfering with primary radicals of CCl4 (•CCl3/CCl3OO•).
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影响因子：
3.3
作者：
NAGABABU, E;SESIKERAN, B;LAKSHMAIAH, N
通讯作者：
LAKSHMAIAH, N
DOI：
10.1080/j157v04n04_03
发表时间：
2004-01-01
期刊：
Journal of Herbal Pharmacotherapy
影响因子：
--
作者：
Singh, U. P.;Singh, D P.;Singh, R. B.
通讯作者：
Singh, R. B.
影响因子：
4.2
作者：
Scalbert, A;Williamson, G
通讯作者：
Williamson, G
影响因子：
15.9
作者：
KARMEN, A;WROBLEWSKI, F;LADUE, JS
通讯作者：
LADUE, JS
影响因子：
3.3
作者：
NAGABABU, E;LAKSHMAIAH, N
通讯作者：
LAKSHMAIAH, N