ISOLATION AND CHARACTERIZATION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-IIB GENES FROM THE NURSE SHARK
ISOLATION AND CHARACTERIZATION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-IIB GENES FROM THE NURSE SHARK
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DOI：
10.1073/pnas.91.1.262
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发表时间：
1994-01-04
影响因子：
11.1
通讯作者：
WEISSMAN, IL
中科院分区：
文献类型：
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作者：
BARTL, S;WEISSMAN, IL
主要组织相容性复合体（MHC）包含一组连锁基因，其编码参与小肽抗原结合的细胞表面蛋白，用于随后被T淋巴细胞识别。MHC蛋白具有共同的结构特征以及在抗原结合中起作用的多态性残基的存在和位置。为了比较这些分子的结构和深入了解它们的进化，我们已经从护士鲨Ginglymostoma cirratum分离出两个MHC IIB类基因。两个克隆，最有可能是等位基因，编码的蛋白质不同的13个氨基酸位于推定的抗原结合裂缝。蛋白质结构和多态性残基的位置与它们的哺乳动物对应物相似。虽然这些基因似乎编码一个典型的MHC蛋白，没有T细胞介导的反应已被证明在软骨鱼。护士鲨代表了最原始的生物体，其中IIA类[Kasahara，M.，Vazquez，M.，佐藤，K.，McKinney，E. C. & Flajnik，M. F.等人（1992）Proc. Acad. Sci USA 89，6688-6692]和IIB类基因，推测编码α/β异二聚体，已经被分离。
The major histocompatibility complex (MHC) contains a set of linked genes which encode cell surface proteins involved in the binding of small peptide antigens for their subsequent recognition by T lymphocytes. MHC proteins share structural features and the presence and location of polymorphic residues which play a role in the binding of antigens. In order to compare the structure of these molecules and gain insights into their evolution, we have isolated two MHC class IIB genes from the nurse shark, Ginglymostoma cirratum. Two clones, most probably alleles, encode proteins which differ by 13 amino acids located in the putative antigen-binding cleft. The protein structure and the location of polymorphic residues are similar to their mammalian counterparts. Although these genes appear to encode a typical MHC protein, no T-cell-mediated responses have been demonstrated in cartilaginous fish. The nurse shark represents the most phylogenetically primitive organism in which both class IIA [Kasahara, M., Vazquez, M., Sato, K., McKinney, E. C. & Flajnik, M. F. (1992) Proc. Natl. Acad. Sci USA 89, 6688-6692] and class IIB genes, presumably encoding the alpha/beta heterodimer, have been isolated.