External Volume Expansion Up-Regulates CXCL12 Expression and Enhances Mesenchymal Stromal Cell Recruitment toward Expanded Prefabricated Adipose Tissue in Rats
External Volume Expansion Up-Regulates CXCL12 Expression and Enhances Mesenchymal Stromal Cell Recruitment toward Expanded Prefabricated Adipose Tissue in Rats
复制标题
外部体积扩张上调 CXCL12 表达并增强间充质基质细胞向大鼠扩张预制脂肪组织的募集
DOI：
10.1097/prs.0000000000004217
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发表时间：
2018-04-01
影响因子：
3.6
通讯作者：
Dong，Ziqing
中科院分区：
文献类型：
--
作者：
Qin，Zijin;Cai，Junrong;Dong，Ziqing
背景：体外容量扩张装置对脂肪组织再生是有效的。然而，外部体积扩张装置诱导脂肪组织再生的详细机制尚不清楚。方法：采用体外体积扩张装置构建大鼠预制脂肪组织扩张模型。采用酶联免疫吸附法检测局部渗出液和血清中CXCL12的表达水平，采用免疫组织化学法检测脂肪组织中CXCL12的表达。将荧光染料（CM-DiI）标记的骨髓间充质间质细胞和经CXCR4拮抗剂AMD3100预处理的标记间充质间质细胞移植到大鼠体内，并通过荧光成像追踪。结果：局部渗出液和血清中CXCL12水平分别在体外容量扩张器应用后2天和7天达到峰值。体外容量扩增组CXCL12+细胞计数明显高于对照组。这些CXCL12+细胞主要呈柱状或立方状，并开始表达过氧化物酶体增殖物激活受体&ggr；在第9天。cm - dii标记的间充质间质细胞被成功募集到扩张的预制脂肪组织中，这一过程被CXCR4拮抗剂AMD3100部分抑制。这些募集的cm - dii标记间充质间质细胞在CXCL12+柱状细胞中被发现。结论：体外体积扩张装置可提高CXCL12的表达水平，尤其是在柱状细胞和立方细胞中。CXCL12/CXCR4通路参与募集循环间充质基质细胞参与脂肪再生。这些发现可能揭示了外部体积膨胀的机制，并为这些装置的改进提供了见解。
Background: External volume expansion devices are effective for adipose tissue regeneration. However, the detailed mechanisms by which external volume expansion devices induce adipose tissue regeneration remain unclear. Methods: An external volume expansion device was used to construct expanded prefabricated adipose tissue in a rat model. CXCL12 levels in local exudate and serum were measured by enzyme-linked immunosorbent assay, and CXCL12 expression in adipose tissue was assessed immunohistochemically. Fluorescent dye (CM-DiI)–labeled bone marrow–derived mesenchymal stromal cells and labeled mesenchymal stromal cells pretreated with the CXCR4 antagonist AMD3100 were transplanted into rats and tracked in vivo by fluorescence imaging. Results: CXCL12 levels in local exudate and serum peaked 2 and 7 days, respectively, after external volume expansion device application. CXCL12+ cell counts were significantly higher in the external volume expansion than in the control group. These CXCL12+ cells were mainly columnar or cuboidal and began to express peroxisome proliferator-activated receptor &ggr; on day 9. CM-DiI–labeled mesenchymal stromal cells were successfully recruited to the expanded prefabricated adipose tissue, a process partly inhibited by the CXCR4 antagonist AMD3100. These recruited CM-DiI–labeled mesenchymal stromal cells were found among the CXCL12+ columnar cells. Conclusions: External volume expansion devices enhance CXCL12 expression levels, especially in columnar and cuboidal cells. The CXCL12/CXCR4 pathway is involved in recruiting circulating mesenchymal stromal cells to participate in adipose regeneration. These findings may reveal the mechanism underlying external volume expansion and provide insights into the refinement of these devices.