Studies on the technique of vitreous fluorophotometry.
Studies on the technique of vitreous fluorophotometry.
复制标题
玻璃体荧光光度测定技术的研究。
DOI：
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复制
发表时间：
1982
影响因子：
4.4
通讯作者：
M. E. Johnson
中科院分区：
文献类型：
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作者：
R. Zeimer;J. Cunha;M. E. Johnson
我们研究了玻璃体荧光光度法（VF）的伪影来源，VF是一种用于评估血视网膜屏障通透性的技术。通过在模型眼中进行体外测量和在人体中进行体内读数，我们研究了前房和视网膜中不同浓度的荧光素对玻璃体中进行的测量的影响。在临床条件下设定了人为效应的上限，并提出了一种解释人为效应的方法。特别注意的是深度分辨率，这是作为被扫描的区域，光束宽度和探头尺寸的函数进行了研究。此外，初步的结果表明，一个程序来考虑光衰减的透镜是可行的。看来，可靠的定量结果，可以通过VF，提供的伪影是公认的，最小化，并纠正。
We examined the sources of artifacts of vitreous fluorophotometry (VF), a technique used to assess the permeability of the blood-retinal barrier. By performing in vitro measurements in a model eye and in vivo readings in humans, we investigated the influence of various concentrations of fluorescein in the anterior chamber and in the retina on the measurements performed in the vitreous. Upper limits for the artifactual effects are set under clinical conditions, and a way to account for them is proposed. Special attention is given to depth resolution, which was investigated as a function of the region being scanned, the beam width, and the probe size. Further, preliminary results indicate that a procedure to account for light attenuation by the lens is feasible. It appears that reliable quantitative results can be obtained by VF, provided that the artifacts are acknowledged, minimized, and corrected.