Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies.
Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies.
复制标题
对大鼠 IgG1、IgG2a 和 IgG2b 亚类以及 kappa 链单型和同种异型决定簇具有特异性的单克隆抗体：与大鼠单克隆抗体配合使用的试剂。
DOI：
10.1089/hyb.1.1982.1.257
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Sanchez-Madrid,F
中科院分区：
文献类型：
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作者：
Springer,TA;Bhattacharya,A;Cardoza,JT;Sanchez-Madrid,F
将免疫SJL小鼠脾细胞与NSI骨髓瘤细胞融合，获得小鼠抗大鼠IgG的单克隆抗体。用~（125）I标记了7种单克隆抗体，并以一组大鼠单克隆抗体作为抑制剂和靶抗原，在软孔板和间接细胞结合试验中研究了它们的特异性和亲合力。所有单克隆抗体均选择4 × 107至≥ 2 × 109 M −1的高亲合力。4株单抗为亚类特异性单抗。RG 11/39、RG 7/1和RG 7/11分别对IgG 1、IgG 2a和IgG 2b的Fc'区具有绝对特异性。RG 9/6对IgG 2a的Fab'区显示特异性，但与IgG 2c的交叉反应具有较低的亲合力。三种单克隆抗体与大鼠κ链反应。RG 7/9定义了单型（共同）κ链决定簇。RG 11/15和RG 7/7分别对等位基因kappa la和kappa lb决定簇具有特异性。单型和kappa la同种异型决定簇在地形上是分开的。所述抗体可用作间接细胞结合测定中的筛选试剂。它们具有与亲和纯化的兔抗大鼠IgG相似的灵敏度和更明确的特异性。它们不与小鼠或人IgG发生交叉反应，因此特别适合用于大鼠抗小鼠或抗人单克隆抗体的配套试剂。RG 7/7单克隆抗体与叙利亚仓鼠IgG有较强的交叉反应。
Mouse monoclonal antibodies to rat IgG were obtained by fusion of immune SJL mouse spleen cells to NSI myeloma cells. Seven monoclonal antibodies have been labeled with125I and studied as to specificity and avidity by using a panel of rat monoclonal antibodies both as inhibitors and target antigens in soft well plate and indirect cell binding assays. All MAb were selected for high avidity of 4 × 107to ≥ 2 × 109M−1. Four MAb were subclassspecific. RG11/39, RG7/1, and RG7/11 were absolutely specific for the Fc' region of IgGl, IgG2a, and IgG2b, respectively. RG9/6 showed specificity for the Fab' region of IgG2a but crossreacted with lower avidity with IgG2c. Three MAb reacted with rat kappa chains. RG7/9 defined a monotypic (common) kappa chain determinant. RG11/15 and RG7/7 were specific for allelic kappa la and kappa lb determinants, respectively. The monotypic and kappa la allotypic determinants are topographically separated. The antibodies can be used as screening reagents in indirect cell binding assays. They have sensitivity similar to affinity-purified rabbit anti-rat IgG and more defined specificity. They do not crossreact with mouse or human IgG, making them particularly suitable companion reagents for rat anti-mouse or anti-human MAb. One Mab, RG7/7, strongly crossreacts with Syrian hamster IgG.