Pulsed Radiofrequency Application Reduced Mechanical Hypersensitivity and Microglial Expression in Neuropathic Pain Model
Pulsed Radiofrequency Application Reduced Mechanical Hypersensitivity and Microglial Expression in Neuropathic Pain Model
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DOI：
10.1111/j.1526-4637.2012.01453.x
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发表时间：
2012-09-01
期刊：
影响因子：
3.1
通讯作者：
Lee, Dong-Gyu
中科院分区：
文献类型：
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作者：
Park, Hea-Woon;Ahn, Sang-Ho;Lee, Dong-Gyu
Objective.脉冲射频（PRF）手术已在临床实践中用于治疗无神经元损伤的慢性神经性疼痛。本研究的目的是研究在神经病理性疼痛模型中，在背根神经节（DRG）上应用PRF后疼痛反应和胶质细胞表达的变化。设计通过单侧L5脊神经结扎（SNL）并在结扎的远侧横断来制备神经性疼痛模型（14只雌性Sprague-Dawley [SD]大鼠; 200 - 250 g）。在术后第9天（POD 9）建立了后爪的机械和冷超敏反应。然后将大鼠随机分配到PRF（+）和PRF（-）组。此外，在POD 10时，在同侧DRG上施加PRF（2个脉冲串/秒，持续时间= 20毫秒，输出电压= 45 V）180秒，最高温度为42 ℃。在PRF后的12天内测试疼痛行为。免疫组化法检测脊髓胶质细胞表达的变化。结果从单次PRF手术后第1天开始，观察到PRF（+）组的机械超敏反应显著降低，并在随后的12天内保持。与PRF（-）组相比，同侧脊髓背角OX 42的免疫反应性也降低。然而，冷超敏反应和胶质细胞酸性蛋白（GFAP）的免疫反应性在背角没有受到影响的PRF程序。结论.我们的研究结果表明，机械超敏反应，由L5 SNL诱导，被削弱的PRF程序对同侧DRG。这种镇痛作用可能与脊髓背角小胶质细胞激活减弱有关。
Objective. Pulsed radiofrequency (PRF) procedure has been used in clinical practice for the treatment of chronic neuropathic pain conditions without neuronal damage. The purpose of this study was to investigate the changes in pain response and glial expression after the application of PRF on a dorsal root ganglion (DRG) in a neuropathic pain model. Design. A neuropathic pain model (14 female Sprague-Dawley [SD] rats; 200250 g) was made by a unilateral L5 spinal nerve ligation (SNL) and transection on the distal side of the ligation. The development of mechanical and cold hypersensitivity on the hindpaw was established postoperative day 9 (POD 9). The rats were then randomly assigned to the PRF (+) and the PRF (-) groups. Furthermore, PRF (2 bursts/s, duration = 20 milliseconds, output voltage = 45 V) was applied on the ipsilateral DRG for 180 seconds, with a maximum temperature of 42 degrees C, at POD 10. Pain behaviors were tested throughout the 12 days after PRF. We also examined the changes of the spinal glial expression by immunohistochemistry. Results. Significant reduction of mechanical hypersensitivity in the PRF (+) group was observed from day 1 after a single PRF procedure and was maintained throughout the following 12 days. Immunoreactivity for OX42 in the ipsilateral dorsal horn also decreased compared with that of the PRF (-) group. However, cold hypersensitivity and glial fibrillary acidic protein (GFAP) immunoreactivity in the dorsal horn was not affected by a PRF procedure. Conclusions. Our result demonstrated that the mechanical hypersensitivity, induced by L5 SNL, was attenuated by a PRF procedure on the ipsilateral DRG. This analgesic effect may be associated with an attenuation of the microglial activation in the dorsal horn.