Glutamine protects against LPS-induced inflammation via adjusted NODs signaling and enhanced immunoglobulins secretion in rainbow trout leukocytes
Glutamine protects against LPS-induced inflammation via adjusted NODs signaling and enhanced immunoglobulins secretion in rainbow trout leukocytes
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谷氨酰胺通过调整 NOD 信号传导和增强虹鳟白细胞中的免疫球蛋白分泌来防止 LPS 诱导的炎症
DOI：
10.1016/j.dci.2019.05.006
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发表时间：
2019-09-01
影响因子：
2.9
通讯作者：
Wang, Qingchao
中科院分区：
文献类型：
--
作者：
Li, Shan;Guo, Qian;Wang, Qingchao
为研究谷氨酰胺（GLN）对虹鳟鱼免疫应答的影响，从虹鳟鱼头肾中分离白细胞，在添加不同浓度脂多糖（LPS）和GLN的DMEM培养基中培养。LPS显著增加促炎细胞因子的表达，而GLN补充减轻LPS诱导的炎症。+GLN + LPS组白细胞表现出更强的GLN拮抗和催化活性，其信号可通过O-GlcNAc化感受，进而影响LPS与细胞表面的结合（LBP），调节NODs信号。免疫球蛋白（Igs）及其受体（pIgR）的mRNA表达也显着增加后，GLN补充。进一步分析表明，GLN增加了IgM（+）B细胞和IgT（+）B细胞的百分比，同时增加了培养基中IgM和IgT的分泌，从而进一步增加了补体C3的表达，以发挥效应功能。这些结果说明GLN通过调节NODs信号和增加Ig（+）B细胞分泌Ig来调节LPS诱导的炎症反应。
To evaluate effects of glutamine (GLN) on fish immune responses, leukocytes were isolated from head kidney of rainbow trout and cultured in GLN-free DMEM media supplemented with different combinations of lipopolysaccharide (LPS) and GLN. LPS significantly increased expression of pro-inflammatory cytokines, while GLN supplementation alleviated LPS-induced inflammation. Leukocytes in +GLN + LPS group showed more active GLN anabolism and catabolism, which signals could be sensed by O-GlcNAcylation, and then affected LPS binding to cell surface (LBP) and adjusted NODs signaling. The mRNA expression of immunoglobulins (Igs) and their receptor (pIgR) was also significantly increased after GLN supplementation. Further analysis showed that GLN increased the percentage of IgM(+) B cells and IgT(+) B cells, accompanied with the increased IgM and IgT secretion in culture media, which further increased complement C3 expression to perform effector functions. All these results illustrated the regulating mechanism of GLN against LPS-induced inflammation both via adjusted NODs signaling and increased Igs(+) B cells to secrete Igs.