MODELS FOR STUDYING LONG-TERM RECOVERY FOLLOWING FOREBRAIN ISCHEMIA IN THE RAT .2. A 2-VESSEL OCCLUSION MODEL
MODELS FOR STUDYING LONG-TERM RECOVERY FOLLOWING FOREBRAIN ISCHEMIA IN THE RAT .2. A 2-VESSEL OCCLUSION MODEL
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DOI：
10.1111/j.1600-0404.1984.tb07822.x
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发表时间：
1984-01-01
影响因子：
3.5
通讯作者：
SIESJO, BK
中科院分区：
文献类型：
--
作者：
SMITH, ML;BENDEK, G;SIESJO, BK
描述了一种模型，在该模型中，通过双侧颈动脉夹闭结合平均动脉血压降低至50 mm Hg，在用N2O：O2（70：30）麻醉的大鼠中诱导短暂性缺血，后者通过出血或通过出血补充给予曲美泛或酚妥拉明来实现。通过使用插管、氯化琥珀胆碱肌肉麻痹和插入尾动脉和静脉导管，可以诱导可逆性缺血用于长期恢复研究。局部CBF [脑血流量]的放射自显影测量结果显示，该手术将新皮质区、海马和尾壳核的CBF降低至接近零值，许多皮质下区域的流速可变。曲美沙芬或酚妥拉明的管理不影响缺血和缺血后的流量，也没有改变恢复脑电图和感觉诱发反应，但曲美沙芬钝化肾上腺素和去甲肾上腺素血浆浓度的变化。恢复实验表明，10分钟的缺血引起了永久性脑损伤的明显迹象，少数动物发生缺血后癫痫发作，导致动物在癫痫持续状态中死亡。缺血15分钟后，这种变化更加明显，大多数动物死亡。注意到的短的复苏时间解释的事实，该模型诱导接近完全缺血，并且恢复后前脑缺血是严重依赖于在缺血期间的残余流速。
A model is described in which transient ischemia is induced in rats anesthetized with N2O:O2 (70:30) by bilateral carotid artery clamping combined with a lowering of mean arterial blood pressure to 50 mm Hg, the latter being achieved by bleeding, or by bleeding supplemented with administration of trimetaphan or phentolamine. By the use of intubation, muscle paralysis with suxamethonium chloride and insertion of tail arterial and venous catheters, it was possible to induce reversible ischemia for long-term recovery studies. Autoradiographic measurements of local CBF [cerebral blood flow] showed that the procedure reduced CBF in neocortical areas, hippocampus and caudoputamen to near-zero values, flow rates in a number of subcortical areas being variable. Administration of trimethaphane or phentolamine did not affect ischemic and postischemic flow rates, nor did they alter recovery of EEG and sensory-evoked responses, but trimetaphan blunted the changes in plasma concentrations of adrenaline [epinephrine] and noradrenaline [norepinephrine]. Recovery experiments showed that 10 min of ischemia gave rise to clear signs of permanent brain damage, with a small number of animals developing postischemic seizures that led to the death of the animals in status epilepticus. After 15 min of ischemia, such alterations were more pronounced, and the majority of animals died. The short revival times noted are explained by the fact that the model induces near-complete ischemia, and that recovery following forebrain ischemia is critically dependent on residual flow rates during the period of ischemia.