Cellular maturation in human preleukemia.
Cellular maturation in human preleukemia.
复制标题
人类白血病前期的细胞成熟。
作者：
H. Koeffler;D. Golde
我们在液体培养中研究了三名骨髓核型异常突出的白血病前期患者的骨髓细胞，以确定肿瘤克隆是否能够成熟。平行细胞遗传学和细胞学研究在顺序收获的骨髓培养物中进行。所有3例患者的培养均发生成熟，尽管延迟。经过14天的体外培养，大约70%的细胞在形态学、细胞化学和功能上观察到成熟的证据。第21天，85%的细胞成熟。249个中期培养细胞中除2个外，其余均含有肿瘤克隆的细胞遗传学异常。我们的结论是，一些由染色体异常鉴定的白血病前期细胞可以在体外成熟。白血病前期可以看作是“早期白血病”的一种综合征，在早期白血病中，肿瘤克隆已经建立，功能上表现为造血功能低下。造血细胞分化逐渐变得异常，并在急性髓性白血病的几乎完全成熟阻滞中终止。
Bone marrow cells from three preleukemic patients with prominent marrow karyotypic abnormalities were studied in liquid culture to determine if the neoplastic clones were capable of maturation. Parallel cytogenetic and cytologic studies were performed in sequentially harvested bone marrow cultures. Maturation, albeit delayed, occurred in cultures from all three patients. By 14 days of culture in vitro, morphologic, cytochemical, and functional evidence of maturation was observed in about 70% of the cells. By day 21, 85% of the cells were mature by these criteria. All but 2 of 249 metaphases from the cultured cells contained the cytogenetic abnormality of the neoplastic clone. We conclude that some preleukemic cells identified by a chromosomal abnormality can mature in vitro. Preleukemia may be viewed as a syndrome of "early leukemia" in which the neoplastic clone is established and manifested functionally as ineffective hematopoiesis. Hematopoietic cell differentiation becomes progressively abnormal with termination in the nearly complete maturational block characteristic of acute myelogenous leukemia.