Off-line coupling of non-aqueous reversed-phase and silver ion high-performance liquid chromatography-mass spectrometry for the characterization of rice oil triacylglycerol positional isomers
Off-line coupling of non-aqueous reversed-phase and silver ion high-performance liquid chromatography-mass spectrometry for the characterization of rice oil triacylglycerol positional isomers
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DOI：
10.1016/j.chroma.2004.04.063
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发表时间：
2004-07-02
影响因子：
4.1
通讯作者：
Mondello, L
中科院分区：
文献类型：
--
作者：
Dugo, P;Favoino, O;Mondello, L
确定现实世界基质中的三酰甘油 (TAG) 谱相当困难，因为这些化合物具有复杂的组成并且具有相似的物理化学性质。本研究基于高效液相色谱 (HPLC) 对米油样品中 TAG 种类和位置异构体的 TAG 谱进行多维测定。通过非水反相HPLC和银离子(Ag)-HPLC的耦合获得了离线二维系统。对初级柱洗脱液进行分级，然后将感兴趣的级分注入二级柱，从而分离在第一维中未解析的几种 TAG 位置异构体。通过将保留数据与大气压化学电离 (APCI) MS 谱信息相结合来进行峰分配。通过 Ag-HPLC 测定沿甘油主链的脂肪酸分布，并通过 APCI-MS 分析得出的甘油二酯离子比率进行确认。在标准化合物的初步应用中进行了方法验证，测量了精密度和准确度。获得的分析结果表明，稻米油 TAG 遵循植物油的典型分布。 (C) 2004 Elsevier B.V. 保留所有权利。
The determination of the triacylglycerol (TAG) profile in real world matrices is rather difficult as these compounds present a complex composition and are characterized by similar physico-chemical properties. This investigation is based on the high-performance liquid chromatography (HPLC) multidimensional determination of the TAG profile in terms of TAG species and positional isomers in a rice oil sample. The off-line bi-dimensional system was attained through the coupling of non-aqueous reversed-phase HPLC and silver ion (Ag)-HPLC. The primary column eluate was fractionated and the fractions of interest were then injected onto the secondary column, allowing the separation of several TAG positional isomers, unresolved in the first dimension. Peak assignment was carried out by combining retention data with atmospheric pressure chemical ionisation (APCI) MS spectra information. The fatty acid distribution along the glycerol backbone, determined by Ag-HPLC, was confirmed through diglyceride ion ratios derived from APCI-MS analysis. Method validation, where both precision and accuracy were measured, was carried out in preliminary applications on standard compounds. The analytical results obtained show that rice oil TAGs follow a distribution which can be considered typical for vegetable oils. (C) 2004 Elsevier B.V. All rights reserved.