Race-specific elicitors from the Peronospora parasitica / Arabidopsis thaliana pathosystem☆
Race-specific elicitors from the Peronospora parasitica / Arabidopsis thaliana pathosystem☆
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来自寄生霜霉/拟南芥病理系统的种族特异性诱导子☆
DOI：
10.1006/pmpp.2000.0261
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发表时间：
2000
影响因子：
2.7
通讯作者：
A. Slusarenko
中科院分区：
文献类型：
--
作者：
J. Rethage;P. Ward;A. Slusarenko
摘要从拟南芥与peronospora parasitica的相容相互作用中分离出细胞间洗涤液（IWFs）。对IWFs进行过滤灭菌，然后渗透到抗性和易感基因型拟南芥叶片中。采用寄生蜂分离株WELA和NOCO（对col0和RLD有毒性，对l0和RLD有毒性，对l0和RLD无毒性）。在诱导坏死的“互反检查”模式中观察到，来自相容相互作用的IWFs在对“亲本”真菌分离物具有抗性的基因型拟南芥叶片中引起坏死，而在对其敏感的基因型中则没有。方差分析表明，真菌分离物与宿主基因型坏死的交互作用极显著。在亲和互作中，诱导坏死活性与高无性产孢率显著相关。然而，诱导坏死活性与IWF蛋白含量无关。
Abstract Intercellular washing fluids (IWFs) were isolated from compatible interactions ofPeronospora parasitica with Arabidopsis. IWFs were filter-sterilized then infiltrated into leaves of resistant and susceptible Arabidopsis genotypes. P. parasitica isolates WELA (virulent on Landsberg erecta (La-er) and Weiningen (Wei-0) but avirulent on Columbia (Col-0) and RLD) and NOCO (virulent on Col-0 and RLD but avirulent on La-er) were used. A “reciprocal check” pattern of necrosis-induction was observed in which IWFs from a compatible interaction caused necrosis in leaves of the Arabidopsis genotypes resistant to the “parent” fungal isolate but not in those genotypes susceptible to it. Analysis of variance showed that the interaction between fungal isolate and necrosis in host genotypes was highly significant. The necrosis-inducing activity was significantly correlated with a high degree of asexual sporulation in the compatible interaction from which the IWF was isolated. However, the necrosis-inducing activity was not correlated with the protein content of the IWF.