Photoprotection mechanisms under different CO2 regimes during photosynthesis in a green alga Chlorella variabilis
Photoprotection mechanisms under different CO2 regimes during photosynthesis in a green alga Chlorella variabilis
复制标题
绿藻小球藻光合作用过程中不同CO2条件下的光保护机制
DOI：
10.1007/s11120-020-00757-4
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发表时间：
2020
影响因子：
3.7
通讯作者：
Seiji Akimoto
中科院分区：
文献类型：
--
作者：
Yoshifumi Ueno;Ginga Shimakawa;Shimpei Aikawa;Chikahiro Miyake;Seiji Akimoto
含氧光合作用通过电子传递将光能转化为化学能，并利用化学能在卡尔文-本森循环中同化CO2。因此，强光和低CO2条件诱导光合电子传递系统中电子的积累，导致活性氧的形成。为了防止电子的积累，产氧光合生物已经发展了光保护机制，包括非光化学猝灭（NPQ）和交替电子流（AEF）。NPQ和AEF有不同的分子机制，相应的分子演员已被确定和表征使用一个模型绿色藻类莱茵衣藻。相比之下，其他绿色藻类的光保护机制缺乏详细的信息。本研究通过结合脉冲幅度调制荧光、O2释放、稳态和时间分辨荧光光谱分析，探讨了绿色小球藻（Chlorella variabilis）响应CO2的光保护机制。在CO2限制条件下，光系统I和光系统II发生了Δ pH依赖的NPQ。此外，还诱导了O2依赖性AEF。在CO2限制的条件下，碳补充，NPQ放松和捕光叶绿素蛋白复合物II从两个光系统分离。Inc.在不同CO2条件下，O2依赖的AEF和两种光系统的捕光功能的即时转换机制对于维持高效的光合活性可能是重要的。
Oxygenic photosynthesis converts light energy into chemical energy via electron transport and assimilates CO2in the Calvin–Benson cycle with the chemical energy. Thus, high light and low CO2conditions induce the accumulation of electrons in the photosynthetic electron transport system, resulting in the formation of reactive oxygen species. To prevent the accumulation of electrons, oxygenic photosynthetic organisms have developed photoprotection mechanisms, including non-photochemical quenching (NPQ) and alternative electron flow (AEF). There are diverse molecular mechanisms underlying NPQ and AEF, and the corresponding molecular actors have been identified and characterized using a model green algaChlamydomonas reinhardtii. In contrast, detailed information about the photoprotection mechanisms is lacking for other green algal species. In the current study, we examined the photoprotection mechanisms responsive to CO2in the green algaChlorella variabilisby combining the analyses of pulse-amplitude-modulated fluorescence, O2evolution, and the steady-state and time-resolved fluorescence spectra. Under the CO2-limited condition, ΔpH-dependent NPQ occurred in photosystems I and II. Moreover, O2-dependent AEF was also induced. Under the CO2-limited condition with carbon supplementation, NPQ was relaxed and light-harvesting chlorophyll-protein complex II was isolated from both photosystems. InC. variabilis, the O2-dependent AEF and the mechanisms that instantly convert the light-harvesting functions of both photosystems may be important for maintaining efficient photosynthetic activities under various CO2conditions.
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有性细胞分裂和接合-新月藻有性生殖中乳头的形成
DOI：
--
发表时间：
1971
期刊：
影响因子：
--
作者：
T. Ichimura
通讯作者：
T. Ichimura
莱茵衣藻突变体的低温荧光发射光谱和叶绿素-蛋白质复合物：与光系统 I 相关的新叶绿素-a-蛋白质复合物的证据
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
J. Garnier;J. Maroc;D. Guyon
通讯作者：
D. Guyon
影响因子：
7.4
作者：
PALMQVIST, K;SUNDBLAD, LG;SAMUELSSON, G
通讯作者：
SAMUELSSON, G
影响因子：
11.6
作者：
Allorent, Guillaume;Tokutsu, Ryutaro;Finazzi, Giovanni
通讯作者：
Finazzi, Giovanni
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影响因子：
3.7
作者：
A. Miller;G. Espie;D. Bruce
通讯作者：
D. Bruce