Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry
Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry
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DOI：
10.1364/oe.17.022879
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发表时间：
2009-12-07
期刊：
影响因子：
3.8
通讯作者：
Adibi, Ali
中科院分区：
文献类型：
--
作者：
Camp, Charles H., Jr.;Yegnanarayanan, Siva;Adibi, Ali
流式细胞术是一种不断发展的高通量多变量分析工具，其本身提供大小和形态信息。然而，为了获得分子信息，通常需要添加荧光团，这受到光谱重叠、非特异性结合、可用的缀合化学和细胞毒性的限制。分子信息的补充或替代的无标记方法是通过多路相干反斯托克斯拉曼散射（MCARS），其是通过探测分子内的拉曼能量来提供丰富的分子信息的相干非线性光学方法。在这项工作中，我们展示了我们的MCARS流式细胞仪的独特能力，区分流动的颗粒，并讨论系统的性能和可能性。(C)2009年美国光学学会
Flow cytometry is an ever-advancing high-throughput multivariate analysis tool that natively provides size and morphological information. To obtain molecular information, however, typically requires the addition of fluorophores, which are limited by spectral overlap, nonspecific binding, available conjugation chemistries, and cellular toxicity. A complementary or alternative, label-free approach to molecular information is through multiplex coherent anti-Stokes Raman scattering (MCARS), which is a coherent, nonlinear optical method that provides a wealth of molecular information by probing the Raman energies within a molecule. In this work, we demonstrate the unique capability of our MCARS flow cytometer to distinguish flowing particles and discuss system performance capabilities and possibilities. (C) 2009 Optical Society of America