Evaluation of a Newly Designed Microperforated Titanium Membrane with Beta-Tricalcium Phosphate for Guided Bone Regeneration in Dog Mandibles
Evaluation of a Newly Designed Microperforated Titanium Membrane with Beta-Tricalcium Phosphate for Guided Bone Regeneration in Dog Mandibles
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DOI：
10.11607/jomi.6776
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发表时间：
2019-09-01
影响因子：
2
通讯作者：
Izumi, Kenji
中科院分区：
文献类型：
--
作者：
Hasegawa, Hiroshi;Kaneko, Tetsuharu;Izumi, Kenji
目的：评价新设计的激光穿孔纯钛膜用于β -磷酸三钙（β - tcp）引导骨再生的效果，并与现有膜进行比较。材料与方法：选取12只犬（每只4只），随机分为2组（每组6只），制作双侧急性侧脊缺损。每组24个骨缺损再进一步分为5组。各组分别为：(1)F001M0，无框架原型膜+ β - tcp (n = 5)；(2) F001M1，框架+ β - tcp的原型膜（n = 5）；(3) FBS，现有对照膜加β - tcp (n = 5)；(4)对照1，不带膜且仅带盖瓣的β - tcp (n = 5)；(5)对照组2，不处理（无β - tcp和无膜）（n = 4）。在所有使用β - tcp的组中，它都与外周血混合。术后6周或12周对动物进行尸检（每只6只），收集样本并进行放射学、组织学和组织形态学分析。结果：三种膜组中，F001M1组术后6周和12周再生组织和骨体积最大，但组间差异无统计学意义。各膜组骨密度相近。组织学分析显示，6周时激光穿孔部位出现未成熟成纤维细胞，诱导血管形成。此外，在12周时，在原型下替换的钙化骨比在FBS膜下替换的钙化骨多。组织形态学分析显示，F001M1和F001M0组术后12周的钙化骨面积明显大于FBS组（P = 0.021, P = 0.032）。此外，术后12周，原型组的膜下纤维组织面积明显小于FBS组（P = 0.02, P = 0.02）。结论：两种原型膜的效果均不低于FBS膜，表明β - tcp与自体血混合后可促进骨再生和成熟。
Purpose: This study evaluated the efficacy of newly designed, laser-perforated pure titanium membranes for guided bone regeneration using beta-tricalcium phosphate (beta-TCP), and compared them with the existing membrane. Materials and Methods: Bilateral acute lateral ridge defects were created in the mandibles of 12 dogs (four defects per animal), which were then randomly divided into two groups (six dogs each). The twenty-four bone defects in each group were then further divided into five groups. The groups were as follows: (1) F001M0, a prototype membrane without a frame plus beta-TCP (n = 5); (2) F001M1, a prototype membrane with a frame plus beta-TCP (n = 5); (3) FBS, an existing control membrane plus beta-TCP (n = 5); (4) control 1, beta-TCP without membrane and with covering flap only (n = 5); and (5) control 2, no treatment (no beta-TCP and no membrane) (n = 4). In all groups where beta-TCP was used, it was mixed with peripheral blood. The animals were necropsied at 6 or 12 weeks postoperatively (six dogs each), and samples were collected and processed for radiographic, histologic, and histomorphometric analyses. Results: Among the three membrane groups, regenerated tissue and bone volume was greatest in the F001M1 group at both 6 and 12 weeks postoperatively, although differences among groups were not statistically significant. Bone mineral density was similar among the membrane groups. Histologic analysis revealed that immature fibroblasts were present on the laser-perforated portion at 6 weeks, which induced vascularization. In addition, more calcified bone was replaced beneath the prototypes than beneath the FBS membrane at 12 weeks. Histomorphometric analyses revealed that the calcific osseous areas at 12 weeks after surgery were significantly greater in the F001M1 and F001M0 groups than in the FBS group (P = .021, P = .032). Furthermore, the fibrous tissue areas beneath the membrane at 12 weeks postoperatively were significantly smaller in the prototype groups than in the FBS group (P = .02, P = .02). Concluslon: The efficacies of both prototype membranes were not inferior to that of the FBS membrane, indicating that they may facilitate bone regeneration and maturation when beta-TCP mixed with autologous blood is employed.