CHARACTERIZATION OF A DROSOPHILA PROTEIN ASSOCIATED WITH BOUNDARIES OF TRANSCRIPTIONALLY ACTIVE CHROMATIN
CHARACTERIZATION OF A DROSOPHILA PROTEIN ASSOCIATED WITH BOUNDARIES OF TRANSCRIPTIONALLY ACTIVE CHROMATIN
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DOI：
10.1101/gad.5.9.1611
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发表时间：
1991-09-01
影响因子：
10.5
通讯作者：
LIS, JT
中科院分区：
文献类型：
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作者：
CHAMPLIN, DT;FRASCH, M;LIS, JT
我们使用间接免疫荧光的多线染色体来检测52-kD果蝇蛋白质染色质的分布，命名为B52。B52定位于转录活性位点，在高度解聚的热休克位点，可以看到对称地支撑RNA聚合酶II的荧光信号。我们还用体内紫外光交联法研究了B52在果蝇细胞培养的非多线染色体上的分布，发现B52在这里也与转录活性染色质的边界有关。预测的B52的初级氨基酸序列揭示了两个与已知与核酸相互作用的其他蛋白质相似的区域。
We have used indirect immunofluorescence of polytene chromosomes to examine the chromatin distribution of a 52-kD Drosophila protein designated B52. B52 is localized to transcriptionally active loci and, at the highly decondensed heat shock loci, can be seen to bracket the RNA polymerase II fluorescence signals symmetrically. We have also examined the distribution of B52 on nonpolytene chromosomes in Drosophila cell cultures with an in vivo UV cross-linking method and find that, here too, B52 is associated with boundaries of transcriptionally active chromatin. The predicted primary amino acid sequence of B52 reveals two regions with similarities to a number of other proteins known to interact with nucleic acids.