Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue
Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue
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DOI：
10.1177/104063879901100607
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发表时间：
1999-11-01
影响因子：
1.5
通讯作者：
Paul, PS
中科院分区：
文献类型：
--
作者：
Sorden, SD;Harms, PA;Paul, PS
建立了石蜡包埋组织中2型猪圆环病毒（PCV2）的免疫组织化学检测方法。兔接种纯化的PCV2获得多克隆抗血清。抗血清应用于含有与断奶后多系统消耗综合征相一致的病变的猪组织切片，其中PCV2基因组已被原位杂交证实。在所有病例（18/18）中，原位杂交或免疫组织化学检测到的阳性细胞密度和分布相同。免疫组织化学方法比原位杂交更快速，更便宜，因此更适合常规诊断使用。
An immunohistochemical method for the detection of type 2 porcine circovirus (PCV2) in paraffin-embedded tissue was developed. Rabbits were inoculated with purified PCV2 to obtain a polyclonal antiserum. Antiserum was applied to sections of porcine tissue that contained lesions consistent with postweaning multisystemic wasting syndrome and in which PCV2 genome had been demonstrated by in situ hybridization. In all cases (18/18), the density and distribution of positive cells detected by in situ hybridization or immunohistochemistry were identical. The immunohistochemical method is more rapid and less expensive than in situ hybridization and is thus more suitable for routine diagnostic use.