Highly pleiotropic functions of CYP78As and AMP1 are regulated in non-cell-autonomous/organ-specific manners
Highly pleiotropic functions of CYP78As and AMP1 are regulated in non-cell-autonomous/organ-specific manners
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DOI：
10.1093/plphys/kiab067
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发表时间：
2021-02-23
期刊：
影响因子：
7.4
通讯作者：
Kusaba, Makoto
中科院分区：
文献类型：
--
作者：
Nobusawa, Takashi;Kamei, Misaki;Kusaba, Makoto
拟南芥中的细胞色素 P450 CYP78A5/KLUH 预计参与移动信号分子的合成，该信号分子具有与经典植物激素不同的多效性功能。 CYP78A5 在拟南芥中有五个近亲。我们首先研究了它们的功能，重点关注质体时间、叶子大小和叶子衰老。我们的分析表明，CYP78A5 和 CYP78A7 与质体时间和叶片大小有关，CYP78A6 和 CYP78A9 与叶片衰老有关。使用异源启动子的互补分析和表达分析表明，CYP78A 亚型具有共同的生化功能，并通过器官特异性表达进行功能分化。改变的分生组织程序 1 (amp1) 羧肽酶突变体显示出与 cyp78a5 突变体非常相似的表型。使用边界和组织中心特异性启动子的互补分析表明，CYP78A5 和 AMP1 均以非细胞自主方式发挥作用。对多个 cyp78a 突变体以及 cyp78a 和 amp1 突变体之间的杂交的分析表明，AMP1/LIKE AMP1 (LAMP1) 和 CYP78A 亚型在相同的遗传途径中调节质体时长和叶片衰老，而叶片大小是独立调节的。此外，我们在基因表达水平上检测到 CYP78A6/CYP78A9 和 AMP1 之间的反馈调节。这些观察结果提出了 AMP1 和 CYP78A 亚型参与相同移动信号分子的合成的可能性，并表明 AMP1 和 CYP78A 信号通路具有非常密切但复杂的功能关系。
The cytochrome P450 CYP78A5/KLUH in Arabidopsis thaliana is predicted to be involved in the synthesis of a mobile signal molecule that has a pleiotropic function that is distinct from classical phytohormones. CYP78A5 has five close relatives in Arabidopsis. We first investigated their functions, focusing on the plastochron, leaf size, and leaf senescence. Our analyses revealed that CYP78A5 and CYP78A7 are involved in the plastochron and leaf size, and CYP78A6 and CYP78A9 are involved in leaf senescence. Complementation analyses using heterologous promoters and expression analyses suggested that CYP78A isoforms have a common biochemical function and are functionally differentiated via organ-specific expression. The altered meristem program1 (amp1) carboxypeptidase mutant shows a phenotype very similar to that of the cyp78a5 mutant. Complementation analyses using boundary and organizing center-specific promoters suggested that both CYP78A5 and AMP1 act in a non-cell-autonomous manner. Analyses of multiple cyp78a mutants and crosses between cyp78a and amp1 mutants revealed that AMP1/LIKE AMP1 (LAMP1) and CYP78A isoforms regulate plastochron length and leaf senescence in the same genetic pathway, whereas leaf size is independently regulated. Furthermore, we detected feedback regulation between CYP78A6/CYP78A9 and AMP1 at the gene expression level. These observations raise the possibility that AMP1 and CYP78A isoforms are involved in the synthesis of the same mobile signal molecule, and suggest that AMP1 and CYP78A signaling pathways have a very close, albeit complex, functional relationship.