Neonatal Bladder Inflammation Produces Functional Changes and Alters Neuropeptide Content in Bladders of Adult Female Rats
Neonatal Bladder Inflammation Produces Functional Changes and Alters Neuropeptide Content in Bladders of Adult Female Rats
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DOI：
10.1016/j.jpain.2009.07.010
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发表时间：
2010-03-01
期刊：
影响因子：
4
通讯作者：
Ness, Timothy J.
中科院分区：
文献类型：
--
作者：
DeBerry, Jennifer;Randich, Alan;Ness, Timothy J.
新生儿膀胱炎症已被证明会对成人膀胱再炎症产生过敏反应。本研究的目的是探讨新生儿膀胱炎症对成人膀胱功能和结构的影响。雌性 Sprague-Dawley 大鼠在出生后第 14 至 16 天用膀胱内酵母聚糖或单独麻醉进行治疗。在 12 至 16 周龄时，测量排尿频率和膀胱压力图。类似处理的大鼠被切除膀胱以测量膀胱内芥子油后的血浆外渗，用于神经肽分析（降钙素基因相关肽或P物质）或用于详细的组织学检查。新生儿时接受酵母聚糖治疗的大鼠表现出排尿频率增加、排尿量阈值降低、膀胱内施用芥子油后伊文思蓝外渗较多、降钙素基因相关肽和P物质的膀胱总含量较高。相反，由于新生儿酵母聚糖治疗，膀胱组织的厚度、纤维化或肥大细胞没有定量的组织学变化。成年期观察到的泌尿系统功能变化，加上成人膀胱中神经肽含量增加和神经源性血浆外渗，表明新生儿膀胱炎症治疗增强了初级传入神经元的数量、功能和/或神经化学含量。这些数据支持这样的假设，即婴儿期泌尿系统的损伤可能导致成年膀胱过敏的发生。观点：大鼠生命早期的膀胱炎症会产生多种后遗症，包括实验室测量表明与膀胱相关的神经生理学基质发生了改变。人类的一些膀胱疼痛综合征具有相似的特征，因此可能是由相似的机制引起的。 (C) 2010 年，美国疼痛协会
Neonatal bladder inflammation has been demonstrated to produce hypersensitivity to bladder re-inflammation as an adult. The purpose of this study was to investigate the effects of neonatal urinary bladder inflammation on adult bladder function and structure. Female Sprague-Dawley rats were treated on postnatal days 14 to 16 with intravesical zymosan or anesthesia alone. At 12 to 16 weeks of age, micturition frequency and cystometrograms were measured. Similarly treated rats had their bladders removed for measurement of plasma extravasation after intravesical mustard oil, for neuropeptide analysis (calcitonin gene-related peptide or Substance P) or for detailed histological examination. Rats treated with zymosan as neonates exhibited increased micturition frequency, reduced micturition volume thresholds, greater extravasation of Evans blue after intravesical mustard oil administration, and greater total bladder content of calcitonin gene-related peptide and Substance P. In contrast, there were no quantitative histological changes in the thickness, fibrosis, or mast cells of bladder tissue due to neonatal zymosan treatments. Functional changes in urologic systems observed in adulthood, coupled with the increased neuropeptide content and neurogenic plasma extravasation in adult bladders, suggest that the neonatal bladder inflammation treatment enhanced the number, function, and/or neurochemical content of primary afferent neurons. These data support the hypothesis that insults to the urologic system in infancy may contribute to the development of adult bladder hypersensitivity.Perspective: Inflammation of the bladder early in life in the rat has multiple sequelae, including laboratory measures that suggest an alteration of the neurophysiological substrates related to the bladder. Some painful bladder syndromes in humans have similar characteristics and so may be due to similar mechanisms. (C) 2010 by the American Pain Society