Use of lacZ fusions to measure in vivo expression of the first three genes of the Escherichia coli unc operon.
Use of lacZ fusions to measure in vivo expression of the first three genes of the Escherichia coli unc operon.
复制标题
使用 lacZ 融合体测量大肠杆菌 unc 操纵子前三个基因的体内表达。
DOI：
10.1128/jb.171.6.3039-3045.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
Brusilow,WS
中科院分区：
文献类型：
--
作者：
Solomon,KA;Hsu,DK;Brusilow,WS
我们已经构建了与大肠杆菌Unc操纵子的前三个基因的框内lacZ蛋白融合体，该操纵子编码质子转运ATP酶的亚基。我们已经使用这些结构来测量这些基因的相对体内表达。第二和第三个基因，uncB和uncE，编码F0区的a和c亚基，以约1：10的相对水平表达，尽管测量的uncB表达取决于有多少基因与lacZ融合。这些速率与纯化的F1F0复合物中a和c亚基的相对数量（a1：c10）相比是有利的。操纵子的第一个基因uncI的体内表达非常低，至多比uncB的表达低10至20倍。
We have constructed in-frame lacZ protein fusions to the first three genes of the Escherichia coli unc operon, which codes for the subunits of the proton-translocating ATPase. We have used these constructions to measure the relative in vivo expression of these genes. The second and third genes, uncB and uncE, which code for the a and c subunits of the F0 sector, were expressed at relative levels of approximately 1:10, although the measured expression of uncB depended upon how much of the gene was fused to lacZ. These rates compared favorably with the relative numbers of a and c subunits (a1:c10) in the purified F1F0 complex. The in vivo expression of uncI, the first gene of the operon, was very low, at best 10 to 20 times less than the expression of uncB.