Multi-color fluorescence photoswitching in fluorescent diarylethene nanoparticles
Multi-color fluorescence photoswitching in fluorescent diarylethene nanoparticles
复制标题
荧光二芳基乙烯纳米颗粒中的多色荧光光开关
DOI：
10.1117/12.2531350
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Tuyoshi.
中科院分区：
文献类型：
--
作者：
Ishida;Sanae;Kim;Sunnam;Kurihara;Seiji;Fukaminato;Tuyoshi.
多色荧光纳米材料，表现出多种可区分的发射信号是特别有吸引力的，因为它们在柔性全色显示器，在下一代光源，并在探针破译多个生物事件同时潜在的应用。最近，我们发现由光致变色二芳基乙烯（DAE）和荧光苯并噻二唑（BTD）单元组成的光开关荧光NP表现出显着的非线性荧光光开关，由于在密集堆积的NP状态下的有效的分子间FRET过程，其中只有少量的非荧光闭环异构体（猝灭剂）就足以猝灭整个荧光信号。这种独特的性质使我们能够演示高对比度的荧光光开关。在这项研究中，我们试图制备几种光开关NP，它们在荧光单元中具有不同的发射最大值，在光致变色DAE单元的闭环异构体中具有不同的吸收最大值。所有化合物在NP态均表现出极大的荧光猝灭。基于这一性质，我们试图证明顺序的红-绿-蓝（RGB）荧光颜色光开关在多组分光致变色荧光NP含有三个不同的荧光颜色的分子和波长选择性的荧光光开关在两个发射彩色光致变色NP的混合物组成的不同对的光开关单元和荧光单元时，适当波长的光照射。这样的荧光光可切换系统具有用于各种应用的巨大潜力。
Multicolor fluorescent nanomaterials that exhibit multiple distinguishable emission signals are especially attractive due to their potential applications in flexible full-color displays, in next-generation lighting sources, and in probes to decipher multiple biological events simultaneously. Recently, we found that a photoswitchable fluorescent NP composed of a photochromic diarylethene (DAE) and a fluorescent benzothiadiazole (BTD) unit exhibits a remarkable nonlinear fluorescence photoswitching due to the efficient intermolecular FRET process in the densely packed NP state, in which only a small amount of the non-fluorescent closed-ring isomer (quencher) was enough to quench the whole fluorescence signal. This unique property allows us the demonstration of high-contrast multicolor fluorescence photoswitching. In this study, we tried to prepare several photoswitchable NPs, which have a different emission maximum in the fluorescence unit and a different absorption maximum in the closed-ring isomer of the photochromic DAE unit. All compounds showed the giant amplified fluorescence quenching in the NP state. Based on this property, we tried to demonstrate the sequential red-green-blue (RGB) fluorescence color photoswitching in a multicomponent photochromic fluorescent NP containing three different fluorescence-colored molecules and the wavelength-selective multicolor fluorescence photoswitching in a mixture of two emission colored photochromic NPs composed of different pairs of a photoswitching unit and a fluorescence unit upon irradiation with appropriate wavelength of lights. Such multicolor fluorescence photoswitchable systems have great potential for various applications.