SEX DIFFERENCE IN SUBUNIT COMPOSITION OF HEPATIC GLUTATHIONE S-TRANSFERASE IN RATS
SEX DIFFERENCE IN SUBUNIT COMPOSITION OF HEPATIC GLUTATHIONE S-TRANSFERASE IN RATS
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DOI：
10.1093/oxfordjournals.jbchem.a135249
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
KITAGAWA, H
中科院分区：
文献类型：
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作者：
IGARASHI, T;SATOH, T;KITAGAWA, H
雄性大鼠肝细胞质谷胱甘肽S-转移酶（GST）对1,2-二氯-4-硝基苯的活性高于雌性大鼠。对 1-氯-2,4-二硝基苯的酶活性在两性之间没有显着差异。通过亲和柱层析从雄性和雌性大鼠肝细胞质中纯化的GST进行SDS-PAGE[十二烷基硫酸钠-聚丙烯酰胺凝胶电泳]分析表明，两性之间的亚基组成存在显着差异。对于雄性和雌性大鼠的几种GST同工酶，通过色谱聚焦将具有碱性和中性/酸性等电点的同工酶分离成7个分子种类。 GST同工酶的定量比例的性别差异也通过使用抗GST-BL和-AC抗体的免疫滴定得到证实。雌性大鼠肝细胞质中对过氧化氢和氢过氧化异丙苯的谷胱甘肽过氧化物酶（GSH-Px）活性显着高于雄性。在两种类型的GSH-Px中，含硒酶（Se-GSH-Px）和非硒依赖性酶（non-Se-GSH-Px），前者主要是造成酶活性性别差异的原因。 GST（非 Se-GSH-Px）的 GSH-Px 活性在女性中也高于男性。由于BL型GST同工酶对氢过氧化枯烯具有GSH-Px活性，因此雌性肝细胞质中非Se-GSH-Px活性的增加似乎主要是由于同工酶GST-L2和-BL的转移酶活性增加所致。
The activities of hepatic cytosolic glutathione S-transferases (GST) towards 1,2-dichloro-4-nitrobenzene in male rats were higher than those in females. The enzyme activities towards 1-chloro-2,4-dinitrobenzene were not significantly different between the 2 sexes. SDS-PAGE [sodium dodecyl sulfate-polyacrylamide gel electrophoresis] analysis of GST purified from male and female rat hepatic cytosols by affinity column chromatography showed that there was a significant difference in the subunit composition between the 2 sexes. With regard to the several isozymes of GST in male and female rats, isozymes with basic and neutral/acidic isoelectric points were separated into 7 molecular species by chromatofocusing. The sex differences in the quantitative proportions of GST isozymes were also confirmed by immunotitration using anti-GST-BL and -AC antibodies. Glutathione peroxidase (GSH-Px) activities in rat hepatic cytosol towards hydrogen peroxide and cumene hydroperoxide were markedly higher in females than in males. Of the 2 types of GSH-Px, selenoenzyme (Se-GSH-Px) and the Se-independent enzyme (non-Se-GSH-Px), the former was mainly responsible for the sex difference in the enzyme activities. The GSH-Px activity of GST, non-Se-GSH-Px, was also higher in females than that in males. Since GST isozymes of the BL type possess GSH-Px activity towards cumene hydroperoxide, the increased activities of non-Se-GSH-Px in the female hepatic cytosol seemed to be mainly due to the increased transferase activities of the isozymes, GST-L2 and -BL.