Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori.
Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori.
复制标题
不对称随机游走揭示了趋化网络调节幽门螺杆菌鞭毛旋转偏向。
DOI：
10.7554/elife.63936
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发表时间：
2021-01-25
期刊：
影响因子：
7.7
通讯作者：
Lele PP
中科院分区：
文献类型：
--
作者：
Antani JD;Sumali AX;Lele TP;Lele PP
典型的趋化性网络调节细菌鞭毛马达中特定旋转方向的偏好，以帮助细胞向有利的化学环境迁移。幽门螺杆菌的趋化网络如何调节鞭毛功能尚不清楚，这限制了我们对该物种趋化性的理解。在这里，我们确定H.通过分析它们与边界表面的流体动力学相互作用，发现当它们的鞭毛逆时针（顺时针）旋转时，幽门螺杆菌游得更快（更慢）。游泳中的这种不对称性有助于量化旋转偏差。在暴露于化学引诱剂后，偏差降低，细胞倾向于仅以更快的模式游泳。在缺乏关键的趋化蛋白CheY的情况下，偏差为零。反转频率和旋转偏置之间的关系是单峰的。因此，H.幽门螺杆菌的趋化网络似乎调节顺时针旋转的概率，否则逆时针旋转鞭毛，类似于典型的网络。
The canonical chemotaxis network modulates the bias for a particular direction of rotation in the bacterial flagellar motor to help the cell migrate toward favorable chemical environments. How the chemotaxis network in Helicobacter pylori modulates flagellar functions is unknown, which limits our understanding of chemotaxis in this species. Here, we determined that H. pylori swim faster (slower) whenever their flagella rotate counterclockwise (clockwise) by analyzing their hydrodynamic interactions with bounding surfaces. This asymmetry in swimming helped quantify the rotational bias. Upon exposure to a chemo-attractant, the bias decreased and the cells tended to swim exclusively in the faster mode. In the absence of a key chemotaxis protein, CheY, the bias was zero. The relationship between the reversal frequency and the rotational bias was unimodal. Thus, H. pylori’s chemotaxis network appears to modulate the probability of clockwise rotation in otherwise counterclockwise-rotating flagella, similar to the canonical network.