Effect of F-actin and Microtubules on Cellular Mechanical Behavior Studied Using Atomic Force Microscope and an Image Recognition-Based Cytoskeleton Quantification Approach
Effect of F-actin and Microtubules on Cellular Mechanical Behavior Studied Using Atomic Force Microscope and an Image Recognition-Based Cytoskeleton Quantification Approach
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DOI：
10.3390/ijms21020392
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发表时间：
2020-01-02
影响因子：
5.6
通讯作者：
Ren, Juan
中科院分区：
文献类型：
--
作者：
Liu, Yi;Mollaeian, Keyvan;Ren, Juan
细胞骨架形态在调节细胞力学中起着关键作用。特别是，细胞质中的F-肌动蛋白和微管的高度交联和动态的细胞骨架结构直接调节细胞的机械性能。尽管人们已经致力于研究细胞骨架状态与细胞力学性能之间的定性关系，但对于F-肌动蛋白和微管的状态如何影响力学行为，目前还缺乏全面的量化结果。在这项研究中，通过原子力显微镜压痕实验和提出的基于图像识别的细胞骨架量化方法，量化了F-肌动蛋白和微管形态对细胞力学性能的影响。对不同细胞骨架状态的NIH/3T3细胞在不同长度尺度上的杨氏模数和扩散系数进行了量化。结果发现，活的NIH/3T3细胞能够感知并适应F-肌动蛋白和微管的状态：在所有测量的压痕深度，细胞弹性和孔道弹性都与F-肌动蛋白和微管的解聚程度密切相关。此外，F-肌动蛋白和微管在影响细胞力学行为中的定量效应的意义是深度相关的。
Cytoskeleton morphology plays a key role in regulating cell mechanics. Particularly, cellular mechanical properties are directly regulated by the highly cross-linked and dynamic cytoskeletal structure of F-actin and microtubules presented in the cytoplasm. Although great efforts have been devoted to investigating the qualitative relation between the cellular cytoskeleton state and cell mechanical properties, comprehensive quantification results of how the states of F-actin and microtubules affect mechanical behavior are still lacking. In this study, the effect of both F-actin and microtubules morphology on cellular mechanical properties was quantified using atomic force microscope indentation experiments together with the proposed image recognition-based cytoskeleton quantification approach. Young's modulus and diffusion coefficient of NIH/3T3 cells with different cytoskeleton states were quantified at different length scales. It was found that the living NIH/3T3 cells sense and adapt to the F-actin and microtubules states: both the cellular elasticity and poroelasticity are closely correlated to the depolymerization degree of F-actin and microtubules at all measured indentation depths. Moreover, the significance of the quantitative effects of F-actin and microtubules in affecting cellular mechanical behavior is depth-dependent.