Role of organic/sulfide ratios on competition of DNRA and denitrification in a co‑driven sequencing biofilm batch reactor
Role of organic/sulfide ratios on competition of DNRA and denitrification in a co‑driven sequencing biofilm batch reactor
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有机物/硫化物比例对联驱测序生物膜间歇式反应器中 DNRA 竞争和反硝化的作用
DOI：
10.1007/s11356-021-17058-5
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发表时间：
2021
影响因子：
5.8
通讯作者：
Jiaojie He
中科院分区：
文献类型：
--
作者：
Xiaoling Li;Jianqiang Zhao;Yuhao Zhang;Jiaojie He
在硝酸盐还原过程中，反硝化和异化硝态还原成铵是两个相互竞争的途径。本研究在测序生物膜间歇式反应器（SBBR）中研究了从8:1到2:4的一系列C/S比，以确定还原剂（硫化物和乙酸酯）对其竞争的作用。结果表明，无论是富有机体系还是富硫化物体系，高电子体系中DNRA的比例都有所增加。当C/S比为2:3时，DNRA比率最高，达16.4%。过量的电子供体，特别是硫化物，在有限的硝酸盐环境中有利于DNRA。此外，较高的还原环境有利于DNRA，特别是当ORP低于- 400 mV时。16S rRNA基因测序分析表明，geobacteri可能是富有机系统中DNRA过程的重要参与者，而desulfomicrobiumi可能是富硫化物系统中DNRA过程的优势菌。DNRA的培养丰富了常规反硝化系统的氮转化途径，加深了对低碳氮比下脱氮的认识。图形抽象
Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two competing pathways in nitrate-reducing process. In this study, a series of C/S ratios from 8:1 to 2:4 were investigated in a sequencing biofilm batch reactor (SBBR) to determine the role of reducers (sulfide and acetate) on their competition. The results showed that the proportion of DNRA increased in high electron system, either in organic-rich system or in sulfide-rich system. The highest DNRA ratio increased to 16.4% at the C/S ratio of 2:3. Excess electron donors, particularly sulfide, were favorable for DNRA in a limited nitrate environment. Moreover, a higher reductive environment could facilitate DNRA, especially, when ORP was lower than − 400 mV in this system. 16S rRNA gene sequencing analysis demonstrated thatGeobactermight be the important participant involved in DNRA process in organic-rich system, whileDesulfomicrobiummight be the dominant DNRA bacteria in sulfide-rich system. DNRA cultivation could enrich nitrogen conversion pathways in conventional denitrification systems and deepen the insight into nitrogen removal at low C/N.Graphical abstract