Expression and Purification of SH2 Domains Using Baculovirus Expression System
Expression and Purification of SH2 Domains Using Baculovirus Expression System
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DOI：
10.1007/978-1-4939-6762-9_11
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发表时间：
2017-01-01
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影响因子：
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通讯作者：
Machida, Kazuya
中科院分区：
文献类型：
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作者：
Ogiue-Ikeda, Mari;Machida, Kazuya
在细菌中表达的重组蛋白有时是不溶性的、聚集的和不正确折叠的。对于那些在细菌中不溶的Src同源2（SH 2）结构域，杆状病毒-昆虫细胞表达系统可以是产生可溶性和功能活性蛋白的替代方案。我们描述了一种使用Bac-to-Bac杆状病毒表达系统克隆和纯化GST标记的SH 2结构域的方案。
Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble and functionally active proteins. We describe a protocol for cloning and purification of GST-tagged SH2 domains using the Bac-to-Bac baculovirus expression system.