DIFFERENTIAL EXPRESSION OF ALPHA-THYROID AND BETA-THYROID HORMONE RECEPTOR GENES IN RAT-BRAIN AND PITUITARY
DIFFERENTIAL EXPRESSION OF ALPHA-THYROID AND BETA-THYROID HORMONE RECEPTOR GENES IN RAT-BRAIN AND PITUITARY
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DOI：
10.1073/pnas.86.18.7250
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发表时间：
1989-09-01
影响因子：
11.1
通讯作者：
WEINBERGER, C
中科院分区：
文献类型：
--
作者：
BRADLEY, DJ;YOUNG, WS;WEINBERGER, C
Multiple thyroid hormone receptor cDNAs have previously been identified in rat and are classified into .alpha. and .beta. subtypes. Alternative splicing of the .alpha. gene gives rise to the functional receptor, rTR.alpha.1, and the non-thyroid hormone-binding isotype, rTR.alpha.2. Recent evidence suggests the .beta. gene encodes two functional receptors, rTR.beta.1, and the pituitary-specific receptor, rTR.beta.2. By using synthetic DNA probes common to rTR.beta. transcripts and specific for rTR.alpha.1 and rTR.alpha.2 mRNAs, we mapped the expression of these transcripts in adult rat brain and pituitary by hybridization histochemistry. We also localized mRNAs encoding the putative nuclear receptor REV-ErbA.alpha., a portion of which is derived from the opposite strand of the rTR.alpha. gene. rTR.alpha.1 and rTR.alpha.2 transcripts were widely distributed in a similar, if not identical, pattern. Highest levels of rTR.alpha.1 and rTR.alpha.2 transcripts were found in the olfactory bulb, hippocampus, and granular layer of the cerebellar cortex. REV-ErbA.alpha. and rTR.beta. mRNAs were found in more restricted patterns of expression distinct from those of rTR.alpha.1 and rTR.alpha.2. REV-ErbA.alpha. mRNA was highest in the neocortex. High levels of rTR.beta. transcripts in the anterior pituitary and the parvocellular part of the paraventricular hypothalamic nucleus suggest rTR.beta. gene products may mediate thyroid hormone feedback regulation of thyroid-stimulating hormone and thyrotropin-releasing hormone. Our results identify nuclei and structures in the mammalian central nervous system in which regulation of gene expression by specific thyroid hormone receptor subtypes may occur.
