Characterization of an anti-Listeria bacteriocin produced by Lactobacillus plantarum LB-B1 isolated from koumiss, a traditionally fermented dairy product from China
Characterization of an anti-Listeria bacteriocin produced by Lactobacillus plantarum LB-B1 isolated from koumiss, a traditionally fermented dairy product from China
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从马奶酒（一种来自中国的传统发酵乳制品）中分离出的植物乳杆菌 LB-B1 产生的抗李斯特菌细菌素的表征
DOI：
10.1016/j.foodcont.2010.12.007
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发表时间：
2011-07-01
期刊：
影响因子：
6
通讯作者：
Zhang, Liebing
中科院分区：
文献类型：
--
作者：
Xie, Ying;An, Haoran;Zhang, Liebing
植物乳杆菌LB-B1是从传统发酵乳制品酸奶中分离到的一株细菌素，对李斯特氏菌、乳杆菌、链球菌、肠球菌、链球菌和埃希氏菌都有抑制作用。儿茶素LB-B1对蛋白水解酶敏感，但在pH 2.0-10.0和耐热性(121℃下15min)范围内稳定。它不会附着在生产细胞的表面。然而，在耐药细胞和敏感细胞上都观察到了吸附。细菌素的产生始于早期的指数阶段，并在早期的稳定阶段达到最大。这一结果表明，儿茶素LB-B1是一种主要代谢物。它的作用方式是杀菌，如对单核细胞增多性李斯特菌54002的测定。经Tricine-SDS-PAGE分析，儿茶素B-B1的相对分子质量在2.5~6.5 kDa之间。此外，编码小孢子菌素LB-B1的基因簇与编码小孢子菌素PA-1的操纵子有99.8%的同源性，表明这两个细菌素是相同的。(C)2010爱思唯尔有限公司。保留所有权利。
Lactobacillus plantarum LB-B1, isolated from a traditionally fermented dairy product koumiss, produced a bacteriocin active against strains of Listeria, Lactobacillus, Streptococcus, Enterococcus, Pediococcus and Escherichia. Pediocin LB-B1 was sensitive to proteolytic enzymes, but stable between pH 2.0-10.0 and heat resistant (15 min at 121 degrees C). It did not adhere to the surface of the producer cells. However, adsorption to both resistant and sensitive cells was observed. Production of the bacteriocin started at the early exponential phase and reached its maximum at the early stationary phase. This result suggested that pediocin LB-B1 was produced as a primary metabolite. Its mode of action was bactericidal, as determined against Listeria monocytogenes 54002. Pediocin LB-B1 was estimated between 2.5 kDa and 6.5 kDa by tricine-SDS-PAGE. Furthermore, the gene cluster encoding pediocin LB-B1 showed 99.8% homology with the operon encoding pediocin PA-1, suggesting that the two bacteriocins are identical. (C) 2010 Elsevier Ltd. All rights reserved.