Repeated exposure to low-dose diesel exhaust after allergen challenge exaggerates asthmatic responses in mice
Repeated exposure to low-dose diesel exhaust after allergen challenge exaggerates asthmatic responses in mice
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DOI：
10.1016/j.clim.2006.08.003
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发表时间：
2006-11-01
影响因子：
8.6
通讯作者：
Sugawara, Isamu
中科院分区：
文献类型：
--
作者：
Matsumoto, Aki;Hiramatsu, Kumiko;Sugawara, Isamu
背景：柴油废气颗粒与过敏原一起作为辅助剂增强IgE反应，诱导细胞因子/趋化因子和粘附分子的表达，增加气道的超反应性（AHR）。由于大多数研究都是在致敏和过敏原挑战期间将动物暴露于柴油废气中，因此尚不清楚柴油废气（DE）暴露是否会夸大哮喘动物的气道反应。目的：研究低剂量DE暴露对哮喘小鼠AHR及变应性气道炎症的影响。方法：腹腔注射卵清蛋白致敏BALB/c小鼠，鼻内注射卵清蛋白致敏BALB/c小鼠。它们暴露于低剂量DE，每天7小时，每周5天，持续12周。采用全身容积描记术、支气管肺泡灌洗细胞分析及肺细胞因子基因表达评价对甲胆碱的AHR。结果：哮喘小鼠重复暴露于低剂量DE可导致AHR和几种促哮喘细胞因子/趋化因子的基因表达增加，但随着持续暴露于DE，这些影响迅速消退。结论：卵清蛋白刺激后重复暴露于低剂量DE可加重小鼠的过敏反应，但持续暴露于DE不会延长这种影响。(c) 2006爱思唯尔公司版权所有。
Background: In conjunction with allergens, diesel exhaust particles act as an adjuvant to enhance IgE responses, inducing expression of cytokines/chemokines and adhesion molecules, and increasing airway hyper-responsiveness (AHR). As most studies were designed to expose animals to diesel exhaust throughout the periods of both sensitization and allergen challenge, it remains unclear whether diesel exhaust (DE) exposure exaggerates airway responses in asthmatic animals.Objective: To study effects of exposure to Low-dose DE on AHR and allergic airway inflammation in asthmatic mice.Methods: BALB/c mice were sensitized by intraperitoneal injection of ovalbumin and challenged by intranasal administration with ovalbumin. They were exposed to tow-dose DE for 7 h/day, 5 days/week, for up to 12 weeks. AHR to methacholine was evaluated by whole-body plethysmography as welt as bronchoalveolar lavage cell analysis and cytokine gene expression in lungs.Results: Repeated exposure of asthmatic mice to low-dose DE resulted in increased AHR and gene expression of several pro-asthmatic cytokines/chemokines, but these effects rapidly subsided with continued exposure to DE.Conclusion: Repeated exposure to tow-dose DE after ovalbumin challenge exaggerates allergic responses in mice, but effects are not prolonged with continuous DE exposure. (c) 2006 Elsevier Inc. All rights reserved.