Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: Validating its traditional usage
Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: Validating its traditional usage
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DOI：
10.1186/1472-6882-9-47
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发表时间：
2009-11-23
影响因子：
--
通讯作者：
Birdi, Tannaz
中科院分区：
文献类型：
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作者：
Brijesh, S.;Daswani, Poonam;Birdi, Tannaz
背景：艾格尔·马梅洛斯（L.）由于其多种药用特性，科雷亚已被广泛用于印度医学的土著系统。然而，尽管其传统用途是作为抗腹泻药，但关于其在感染性腹泻中的作用方式的信息有限。因此，我们评估了柑桔未成熟果肉的热水提取物（煎剂）的抗菌活性和对感染性腹泻的各方面致病性的影响。方法：对其抗菌、抗心绞痛、抗轮状病毒活性进行评价。评估汤剂对肠致病性大肠杆菌的粘附作用以及肠侵袭性大肠杆菌和福氏志贺氏菌对HEp-2细胞的侵袭作用，作为其定殖效果的衡量指标。采用GM1酶联免疫吸附法观察其对大肠杆菌热不稳定毒素（LT）和霍乱毒素（CT）产生的影响及其与神经节苷单唾液酸受体（GM1）结合的影响，采用乳鼠法观察其对大肠杆菌热稳定毒素（ST）产生和作用的影响。结果：该煎剂对贾第鞭毛虫和轮状病毒有一定的杀灭作用，但对6种细菌的活性均无影响。它能显著降低细菌对HEp-2细胞的粘附和侵袭。提取物还影响了CT的产生以及LT和CT与GM1的结合。结论：蜜瓜生果肉煎煮后，虽然抗菌活性有限，但会影响细菌在肠道上皮的定植和某些肠道毒素的产生和作用。这些观察结果表明，柑橘在传染性腹泻中可能有多种作用方式，从而证实了古印度文献中提到的柑橘，并继续被当地社区用于治疗腹泻疾病。
Background: Aegle marmelos (L.) Correa has been widely used in indigenous systems of Indian medicine due to its various medicinal properties. However, despite its traditional usage as an antidiarrhoeal there is limited information regarding its mode of action in infectious forms of diarrhoea. Hence, we evaluated the hot aqueous extract (decoction) of dried unripe fruit pulp of A. marmelos for its antimicrobial activity and effect on various aspects of pathogenicity of infectious diarrhoea.Methods: The decoction was assessed for its antibacterial, antigiardial and antirotaviral activities. The effect of the decoction on adherence of enteropathogenic Escherichia coli and invasion of enteroinvasive E. coli and Shigella flexneri to HEp-2 cells were assessed as a measure of its effect on colonization. The effect of the decoction on production of E. coli heat labile toxin ( LT) and cholera toxin (CT) and their binding to ganglioside monosialic acid receptor (GM1) were assessed by GM1-enzyme linked immuno sorbent assay whereas its effect on production and action of E. coli heat stable toxin (ST) was assessed by suckling mouse assay.Results: The decoction showed cidal activity against Giardia and rotavirus whereas viability of none of the six bacterial strains tested was affected. It significantly reduced bacterial adherence to and invasion of HEp-2 cells. The extract also affected production of CT and binding of both LT and CT to GM1. However, it had no effect on ST.Conclusion: The decoction of the unripe fruit pulp of A. marmelos, despite having limited antimicrobial activity, affected the bacterial colonization to gut epithelium and production and action of certain enterotoxins. These observations suggest the varied possible modes of action of A. marmelos in infectious forms of diarrhoea thereby validating its mention in the ancient Indian texts and continued use by local communities for the treatment of diarrhoeal diseases.