IDENTIFICATION AND PROPERTIES OF AN ATYPICAL CATALYTIC SUBUNIT (P34(PSK-J3)/CDK4) FOR MAMMALIAN-D TYPE-G1 CYCLINS
IDENTIFICATION AND PROPERTIES OF AN ATYPICAL CATALYTIC SUBUNIT (P34(PSK-J3)/CDK4) FOR MAMMALIAN-D TYPE-G1 CYCLINS
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DOI：
10.1016/0092-8674(92)90360-o
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发表时间：
1992-10-16
期刊：
影响因子：
64.5
通讯作者：
SHERR, CJ
中科院分区：
文献类型：
--
作者：
MATSUSHIME, H;EWEN, ME;SHERR, CJ
小鼠D型细胞周期蛋白与一个催化亚单位(p34PSK-J3)相关，具有不同于已知的细胞周期蛋白依赖性蛋白激酶(CDK)的特性。小鼠p34PSK-J3与p34cdc2、p33cdk2和p36cdk3的氨基酸同源性不到50%，缺乏PSTAIRE基序，不与p13uc1结合。Cyclin D1-p34PSK-J3复合体在G1期在巨噬细胞中蓄积，在S时相下降，而Cyclin D2和D3复合体在增殖的T细胞中形成。虽然在细胞周期蛋白D或PSK-J3免疫沉淀物中未检测到组蛋白H1激酶活性，但体外组装的细胞周期蛋白D-p34PSK-J3复合体稳定地结合和磷酸化视网膜母细胞瘤基因产物(PRb)和Rb样蛋白(P107)，但不与功能不活跃的pRb突变体相互作用。因此，p34PSK-J3是一个受周期蛋白D调节的催化亚基，其作用是RB(而不是H1)激酶。
Murine D type cyclins associate with a catalytic subunit (p34PSK-J3 with properties distinct from known cyclin-dependent kinases (cdks). Mouse p34PSK-J3 shows less than 50% amino acid identity to p34cdc2, p33cdk2, and p36cdk3, lacks a PSTAIRE motif, and does not bind to p13suc1. Cyclin D1-p34PSK-J3 complexes accumulate in macrophages during G1 and decline in S phase, whereas complexes involving cyclins D2 and D3 form in proliferating T cells. Although histone H1 kinase activity is not detected in cyclin D or PSK-J3 immunoprecipitates, cyclin D-p34PSK-J3 complexes assembled in vitro stably bind and phosphorylate the retinoblastoma gene product (pRb) and an Rb-like protein (p107) but do not interact with pRb mutants that are functionally inactive. Thus, p34PSK-J3 is a cyclin D-regulated catalytic subunit that acts as an Rb (but not H1) kinase.