Increased MCP-1, RANTES, and MIP-1 alpha in bronchoalveolar lavage fluid of allergic asthmatic patients
Increased MCP-1, RANTES, and MIP-1 alpha in bronchoalveolar lavage fluid of allergic asthmatic patients
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DOI：
10.1164/ajrccm.153.4.8616572
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发表时间：
1996-04-01
影响因子：
24.7
通讯作者：
Ida, N
中科院分区：
文献类型：
--
作者：
Alam, R;York, J;Ida, N
趋化因子是诱导炎性细胞趋化性的细胞因子。我们研究了支气管肺泡灌洗液（BALF）中的趋化因子的存在，从9例过敏性哮喘患者和6名非吸烟的正常人。沉淀细胞，并使用RNAzol B提取核糖核酸（RNA）。通过酶联免疫吸附试验（ELISA）测定BALF中单核细胞趋化蛋白-1（MCP-1）、巨噬细胞炎性蛋白-1 α（MIP-1 α）和白细胞介素-8（IL-8），MCP-1在正常T细胞活化时受到调节，表达，可能分泌（RANTES）。哮喘患者MCP-1、RANTES和MIP-1 α水平显著高于对照组（p < 0.04）。RANTES和MCP-1的浓度与BAL标本中的淋巴细胞计数相关（r分别为0.61和0.68）。BALF在体外显示嗜酸性粒细胞趋化活性，其被抗RANTES和抗MCP-3抗体阻断。逆转录总细胞RNA，并采用聚合酶链反应（PCR）扩增MCP-1、MCP-3、RANTES、MIP-1 α、IL-8和β-肌动蛋白的互补脱氧核糖核酸（cDNA）。我们发现MCP-1、MCP-3、RANTES、MIP-1 α和IL-8的信使核糖核酸（mRNA）由大多数哮喘和正常受试者的BAL细胞产生。我们的结论是，趋化因子产生于气道，MCP-1，RANTES和MIP-1 α的恢复增加，观察过敏性哮喘患者。
Chemokines are cytokines that induce chemotaxis of inflammatory cells. We studied the presence of chemokines in bronchoalveolar lavage fluid (BALF) obtained from nine allergic asthmatic patients and six nonsmoking normal individuals. The cells were pelleted, and ribonucleic acid (RNA) was extracted by using RNAzol B. BALF was assayed for monocyte chemoattractrant protein-1 (MCP-1), regulated upon activation in normal T cells, expressed, probably secreted (RANTES), macrophage inflammatory protein-1 alpha (MIP-1 alpha) and interleukin-8 (IL-8) by enzyme-linked immunosorbent assay (ELISA). The levels of MCP-1, RANTES, and MIP-1 alpha were significantly higher in the asthma patients than in the control subjects (p < 0.04). The concentrations of RANTES and MCP-1 correlated with the lymphocyte count in the BAL specimens (r = 0.61 and 0.68, respectively). BALF showed eosinophil chemotactic activity in vitro that was blocked by anti-RANTES and anti-MCP-3 antibodies. The total cellular RNA was reverse-transcribed and the complementary deoxyribonucleic acid (cDNA) was amplified with the polymerase chain reaction (PCR) for MCP-1, MCP-3, RANTES, MIP-1 alpha, IL-8, and beta-actin. We found that messenger ribonucleic acids (mRNAs) for MCP-1, MCP-3, RANTES, MIP-1 alpha, and IL-8 were produced by BAL cells from most asthmatic and normal subjects. We conclude that chemokines are produced in the airways, and that an increased recovery of MCP-1, RANTES, and MIP-1 alpha is observed in allergic asthmatic patients.