Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three-Dimensional Fibrin Gel.
Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three-Dimensional Fibrin Gel.
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DOI：
10.1002/sctm.16-0427
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发表时间：
2017-05
影响因子：
6
通讯作者：
Wu WS
中科院分区：
文献类型：
--
作者：
Zhu P;Zhou Y;Wu F;Hong Y;Wang X;Shekhawat G;Mosenson J;Wu WS
肌肉干细胞（MuSC）在体内表现出强大的生肌潜力，从而为肌肉疾病提供了一种有前途的治疗方法。由于成人 MuSC 在有限的肌肉活检中缺乏，因此非常需要体外扩增成体 MuSC 以达到治疗细胞剂量。目前所有的培养系统通常都需要对纯 MuSC 进行分选。在这里，我们开发了一种软三维（3D）鲑鱼纤维蛋白凝胶培养系统，可以选择性地从大块骨骼肌制剂中扩增小鼠 MuSC，无需进行细胞分选，并忠实地保持其在培养中的再生能力。我们的研究建立了一个方便的 MuSC 离体扩增的新平台，从而极大地推进了针对各种肌肉疾病的干细胞疗法。干细胞转化医学 2017；6：1412–1423
Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three‐dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell‐based therapies for various muscle disorders. Stem Cells Translational Medicine 2017;6:1412–1423