LncRNA-p23154 promotes the invasion-metastasis potential of oral squamous cell carcinoma by regulating Glut1-mediated glycolysis
LncRNA-p23154 promotes the invasion-metastasis potential of oral squamous cell carcinoma by regulating Glut1-mediated glycolysis
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LncRNA-p23154通过调节Glut1介导的糖酵解促进口腔鳞状细胞癌的侵袭转移潜力
DOI：
10.1016/j.canlet.2018.07.016
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发表时间：
2018-01-01
期刊：
影响因子：
9.7
通讯作者：
Cheng, Bin
中科院分区：
文献类型：
--
作者：
Wang, Yun;Zhang, Xiaojie;Cheng, Bin
糖酵解的失调已被认为导致细胞耐药信号的改变、增殖和转移。新出现的证据表明，lncRNA在肿瘤细胞的细胞过程中发挥关键作用，包括糖酵解，生长和运动。然而，lncRNA在糖酵解介导的转移中的作用和潜在机制尚未被探索。在这项研究中，我们确定了一种新的lncRNA lnc-p23154，这是与口腔鳞癌患者的转移和促进口腔鳞癌细胞迁移和侵袭在体外和体内。此外，我们发现lnc-p23154还通过促进Glut 1表达参与OSCC糖酵解。拯救lnc-p23154逆转了Glut 1敲低诱导的OSCC细胞迁移和侵袭的抑制。此外，Inc-p23154主要位于细胞核中，并与miR-378 a-3 p的启动子区结合，通过直接靶向其3 'UTR来抑制Glut 1的表达。因此，Inc-p23154可能通过抑制miR-378 a-3 p的转录，在Glut 1介导的糖酵解过程中发挥重要作用，促进OSCC的转移。
The dysregulation of glycolysis has been suggested to lead to alteration of cell drug resistance signals, proliferation and metastasis. Emerging evidence indicates that lncRNAs play a key role in the cellular processes of tumor cells, including glycolysis, growth, and movement. However, the role and potential mechanism of lncRNAs in glycolysis-mediated metastasis has not been explored. In this study, we identified a novel lncRNA lnc-p23154 which is associated with OSCC patient metastasis and the promotion of OSCC cell migration and invasion in vitro and in vivo. Furthermore, we found that lnc-p23154 also participates in OSCC glycolysis by facilitating Glut1 expression. Rescue of lnc-p23154 reversed the suppression of OSCC cell migration and invasion induced by Glut1 knockdown. In addition, Inc-p23154 is mainly located in the nucleus and binds to the promoter region of miR-378a-3p, which represses Glut1 expression by targeting to its 3'UTR directly. Therefore, we concluded that Inc-p23154 may play an important role in Glut1-mediated glycolysis by inhibiting miR-378a-3p transcription and accelerate OSCC metastasis.