Molecular genetic characterization of a locus that contains duplicate Adh genes in Drosophila mojavensis and related species.
Molecular genetic characterization of a locus that contains duplicate Adh genes in Drosophila mojavensis and related species.
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果蝇莫哈文西斯及相关物种中含有重复 Adh 基因的基因座的分子遗传学特征。
DOI：
10.1093/genetics/112.2.295
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发表时间：
1986
期刊：
影响因子：
3.3
通讯作者：
Sullivan,DT
中科院分区：
文献类型：
--
作者：
Mills,LE;Batterham,P;Alegre,J;Starmer,WT;Sullivan,DT
莫哈韦果蝇和其他种类的mulleri亚群含有一个重复的基因编码的酶乙醇脱氢酶（ADH）。使用电泳变体对这两个基因的遗传关系进行的研究表明它们是紧密连锁的。我们克隆了一个13.5kb的D.将mojavensis DNA插入λ载体Charon 30中。该片段含有由约2kb DNA分隔的两个Adh基因。原位杂交后，该克隆与莫哈韦3号染色体上的一个位点杂交。很可能基因在基因组中是串联排列的。两个基因中的一个显示出其结构的复杂性，这表明假基因或基因的一部分的紧密连锁。通过构建基因组DNA的限制性内切酶图谱，比较了五个种的Adh基因座的结构。其中两个物种D。arizonensisandD. mojavensis表达Adh-1在卵巢;其他人没有。在比较这些物种时，很明显，在Adh基因之间的区域有一个或两个插入。这些结构变化之一可能与这些物种进化过程中发生的Adhtissue特异性表达的变化有关。
Drosophila mojavensisand other species of themullerisubgroup contain a duplicate gene encoding the enzyme alcohol dehydrogenase (ADH). Studies on the genetic relationship of the two genes using electrophoretic variants show them to be closely linked. We have cloned a 13.5-kb fragment ofD. mojavensisDNA into the lambda vector, Charon 30. This fragment contains bothAdhgenes separated by approximately 2 kb of DNA. The clone hybridized to a single position on chromosome 3 inD. mojavensisfollowingin situhybridization. It is likely that the genes are tandemly arranged in the genome. One of the two genes shows a complexity in its structure that suggests the close linkage of a pseudogene or part of a gene.—The structure of theAdhlocus in five species of themullerisubgroup have been compared by constructing restriction maps of genomic DNA. Two of these speciesD. arizonensisandD. mojavensisexpressAdh-1in the ovaries; the others do not. In comparing these species it is evident that there has been one or two insertions into the region between theAdhgenes. It is possible that one of these structural changes is related to the change inAdhtissue-specific expression that has occurred during the evolution of these species.