Scanning acoustic microscopy visualizes cytomechanical responses to cytochalasin D
Scanning acoustic microscopy visualizes cytomechanical responses to cytochalasin D
复制标题
扫描声学显微镜可视化对细胞松弛素 D 的细胞力学反应
作者：
J. Bereiter
使用0.9 GHz的扫描声学显微镜（SAM）、荧光显微镜和电子显微镜观察XTH-2细胞（来自非洲爪蟾蝌蚪心脏的细胞系）对细胞松弛素D（CD）的反应。可以通过SAM检测到的对CD的第一反应是图像对比度的损失，表明声阻抗降低约30%。基于用TRITC-鬼笔环肽染色肌动蛋白所揭示的结构变化，并考虑到理论因素，推导出肌动蛋白纤维系统中阻抗降低与张力之间的关系。
The reactions of XTH‐2 cells (line derived from Xenopus laevis tadpole hearts) to cytochalasin D (CD) were followed using scanning acoustic microscopy (SAM) at 0.9 GHz, fluorescence and electron microscopy. The first reaction to CD which can be detected by SAM is a loss of image contrast, indicating a decrease in acoustic impedance of about 30%. Based on structural changes revealed by staining of actin with TRITC‐phalloidin, and taking theoretical considerations into account, a relationship between impedance decrease and tension in the actin fibrillar system is deduced.
细胞松弛素 B 和肌动蛋白凝胶的结构。
DOI：
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发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Maruyama,K;Hartwig,JH;Stossel,TP
通讯作者：
Stossel,TP